Determination of Double-wavelength Multiple of Content in

GONG Wei-hong; ZHAO Xi-xian

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Determination of Double-wavelength Multiple of Content in

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- Determination of Double-wavelength Multiple of Content in
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 14, Pages: 58-60(2010)
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- Published：2010
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GONG Wei-hong, ZHAO Xi-xian. Determination of Double-wavelength Multiple of Content in [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 58-60.
DOI：

GONG Wei-hong, ZHAO Xi-xian. Determination of Double-wavelength Multiple of Content in [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 58-60. DOI：

摘要

目的: 建立双波长HPLC测定山茱萸 Cornus officinalis Sieb. et Zucc.中熊果酸、马钱苷含量的方法。方法: 用十八烷基键合硅胶为固定相Shim-pack VP-ODS柱

以乙腈为流动相A

以0.02%磷酸水为流动相B

进行梯度洗脱

检测波长为210

238 nm。结果: 熊果酸在0.1~2.0 μg线性良好(r=0.999 9)。回收率为99.44%(RSD 0.39%

n=6)。马钱苷在0.08~1.6 μg线性良好(r=0.999 9)。回收率为98.92%(RSD0.75%

n=6)。结论: 本方法简便快速

可同时测定山茱萸中多种成分含量

结果准确。

Abstract

Objective: To establish a dual-wavelength HPLC method for the determination of ursolic acid and loganin from Cornus officinalis. Method: Octadecyl bonded silica stationary phase Shim-pack VP-ODS column was used. Gradient elution was performed with acetonitrile as mobile phase A

0.02% phosphoric acid water as mobile phase B. The detection wavelength was set at 210 nm and 238 nm. Result: UA was linear in the range of 0.1- 2.0 μg

(r=0.999 9). The average recovery was 99.44% (RSD 0.39%

n=6). Loganin was linear in the range of 0.08- 1.6 μg. (r=0.999 9). The average recovery was 98.92% (RSD 0.75%

n=6). Conclusion: The method established was simple

rapid and accurate and could be used for simultaneous determination of ursolic acid and loganin from dogwood.

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Related Author

GONG Wei-hong1

ZHAO Xi-xian2

ZHANG Le

LI Xing-hua

MA Xiao-ya

LEI Jian-lin

LI Yuan-hong

CHEN Ji-yan

Related Institution

Beijing University of Chinese Medicine,School of Chinese Materia Medica

Chongwen District,Beijing Institute for Drug Control

Jiangxi College of Traditional Chinese Medicine

Dept.of Pharmacy,The First Affiliated Hospital of Xinjiang Medical University

Tianjin University of Traditional Chinese Medicine

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