HPLC Fingerprint of Radix Aconiti

LIN Hua; DENG Guang-hai

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HPLC Fingerprint of Radix Aconiti

更新时间：2024-01-04

- HPLC Fingerprint of Radix Aconiti
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 3, Pages: 73-76(2011)
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- Published：2011
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LIN Hua, DENG Guang-hai. HPLC Fingerprint of Radix Aconiti[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 73-76.
DOI：

LIN Hua, DENG Guang-hai. HPLC Fingerprint of Radix Aconiti[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 73-76. DOI：

摘要

目的 :建立不同产地川乌药材的高效液相指纹图谱。方法 :实验以Phenemonex Gemini-NX C18 为色谱柱

流动相乙腈-0.2%乙酸 (浓氨水调pH 10.00)

采用梯度洗脱

流速0.8 mL ·min-1

柱温35 ℃

检验波长235 nm

洗脱时间75 min。结果: 建立了川乌药材HPLC指纹图谱共有模式

标定了12个共有峰

鉴别出6个色谱峰的归属

指纹图谱的相似度基本都在92%以上

通过系统聚类分析

10批不同产地川乌药材可分为3类。结论: 川乌指纹图谱的特征性及专属性强

可结合含量测定用于全面控制川乌的质量。

Abstract

Objective: To establish a method for HPLC-fingerprint of Radix Aconiti from different areas. Method: The fingerprint for Radix Aconiti from different areas was established by HPLC. Gemini-NX C18 column was used at 35 ℃ with acetonitrile-0.2% acetic acid (adjusted to pH 10.0 with ammonia water) as mobile phase by gradient eluting

the flow rate was set at 0.8 mL ·min-1

detection wavelength was set at 235 nm and eluting period was 75 min. Result: HPLC-fingerprint of R.aconiti from different areas was established

which contained 12 common peaks

and six peaks in the fingerprint were identified

most of the similarities were over 92%

10 batches of R.aconiti from different areas were classified into three groups based on hierarchical cluster. Conclusion: Fingerprint established was characteristic and specific

combined with the determination it could be used for the quality control of Radix Aconiti .

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