Study on Extraction and Determination Method of Three Liposoluble Effective Constituents in Bufons Venenum
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Study on Extraction and Determination Method of Three Liposoluble Effective Constituents in Bufons Venenum
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 3, Pages: 69-72(2011)
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Published:2011
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LIU Dong, DU Shou-ying, HE Xiu-feng, et al. Study on Extraction and Determination Method of Three Liposoluble Effective Constituents in Bufons Venenum[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 69-72.
DOI:
LIU Dong, DU Shou-ying, HE Xiu-feng, et al. Study on Extraction and Determination Method of Three Liposoluble Effective Constituents in Bufons Venenum[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 69-72.DOI:
Study on Extraction and Determination Method of Three Liposoluble Effective Constituents in Bufons Venenum
Objective: Study on extract method of liposoluble constituent bufalin
cinobufagin and resibufogenin in Bufons Venenum by orthogonal experiment design and established HPLC method for determination the assay of three effective constituents above. Method: An orthogonal test was adopted in this study. The content of bufalin
cinobufagin and resibufogenin was used as index for optimizing the extraction condition. In established HPLC method
the column:Diamonsil-C\-18 column(4.6 mm×250 mm
5 μm)
mobile phase:acetonitrile-water (55 ∶45)
the flow rate :1.0 mL·min-1
the detection wavelength 296 nm. Result: The best extraction technology was to extract for 120 min with 75 times 95% ethanol in 80 ℃. The calibration curves of bufalin
cinobufagin
resibufogenin were linear and LOD were 14.6
9.0
11.8 ng ·mL-1 respectively. The repeatability and precision of the HPLC method was good. Conclusion: Confirmed the best extract method and established HPLC method to determine the assay of three constituents in Bufons Venenum extraction was simple and accurate.
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