DPPH and ABTS Radical Scavenging Capacity of Elsholtzia rugulosa

GUO Zhi-qin; LV Hai-ning; CHEN Qiao-lian; SHE Gai-mei

您当前的位置：

首页 >

文章列表页 >

DPPH and ABTS Radical Scavenging Capacity of Elsholtzia rugulosa

更新时间：2024-09-23

- DPPH and ABTS Radical Scavenging Capacity of Elsholtzia rugulosa
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 11, Pages: 180-183(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2010
- 稿件说明：
移动端阅览
GUO Zhi-qin, LV Hai-ning, CHEN Qiao-lian, et al. DPPH and ABTS Radical Scavenging Capacity of Elsholtzia rugulosa[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 180-183.
DOI：

GUO Zhi-qin, LV Hai-ning, CHEN Qiao-lian, et al. DPPH and ABTS Radical Scavenging Capacity of Elsholtzia rugulosa[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 180-183. DOI：

摘要

目的 :对野坝子 Elsholtzia rugulosa Hemsl.乙醇提取物的不同极性萃取部位和AB-8大孔树脂柱色谱不同浓度甲醇洗脱部位清除自由基能力进行综合评价。方法 :分别以维生素C和维生素E为对照

采用清除2

2-二苯基苦味酰基苯肼基(2

2-diphenyl-1-picrylhydrazyl

DPPH)和2

2'-连氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基的方法

通过计算半数抑制浓度评价野坝子的乙醇提取物的不同极性萃取部位和AB-8大孔树脂柱色谱不同浓度甲醇洗脱部位的清除自由基能力。结果 :野坝子提取物不同极性萃取部位清除DPPH自由基和ABTS自由基的能力分别为:乙酸乙酯部位> Vit C>正丁醇部位>水部位>石油醚部位>氯仿部位、乙酸乙酯部位>Vit E>正丁醇部位>水部位>氯仿部位>石油醚部位。AB-8大孔树脂柱色谱不同浓度甲醇洗脱部位清除DPPH和ABTS自由基的能力分别为Vit C>50%甲醇部位>70%甲醇部位> 30%甲醇部位> 100%甲醇部位、50%甲醇部位> 70%甲醇部位> 30%甲醇部位> Vit E>100%甲醇部位。结论 :野坝子乙醇提取物的乙酸乙酯萃取部位和30%

50%和70%甲醇洗脱部位都具有很好的清除自由基活性

是一种新型天然抗氧化物质来源。

Abstract

The free radical scavenging capacity of different extracts and macroporous resin column chromatography gradient solutions from ethanol extract of Elsholtzia rugulosa were determined by 2

2-diphenyl-1-picrylhydrazyl (DPPH) and improved 2

2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS) assay

with Vit C and Vit E as the control. The free radical scavenging capacity on DPPH and ABTS of different extracts of E. rugulosa followed by ethyl acetate>Vit C > n -butanol>aqueous solution>petroleum ether>chloroform and ethyl acetate>Vit E > n -butanol>aqueous solution>chloroform>petroleum ether

respectively. The free radical scavenging capacity on DPPH and ABTS of macroporous resin column chromatography gradient solutions were Vit C>50% MeOH>70% MeOH>30% MeOH>100% MeOH and 50% MeOH>70% MeOH>30% MeOH > Vit E >100% MeOH. The ethyl acetate extracts and 30%

50% and 70%MeOH solutions exhibited strong antioxidant activity. It indicated as a new source of natural antioxidants.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

DPPH and ABTS Radical Scavenging Capacity of Elsholtzia rugulosa

Related Author

GUO Zhi-qin1

LV Hai-ning1

CHEN Qiao-lian2

SHE Gai-mei1

Related Institution

Department of Traditional Chinese Medicine Chemistry,Chinese Pharmacy,Beijing University of Chinese Medicine

China Agricultural University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰