Determination of Quercitrin from Leaves by Capillary-zone Electrophoresis
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Determination of Quercitrin from Leaves by Capillary-zone Electrophoresis
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 11, Pages: 73-75(2010)
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Published:2010
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DING Hong-mei, SHENG Zhen-hua, GE Er-ning. Determination of Quercitrin from Leaves by Capillary-zone Electrophoresis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 73-75.
DOI:
DING Hong-mei, SHENG Zhen-hua, GE Er-ning. Determination of Quercitrin from Leaves by Capillary-zone Electrophoresis[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 73-75.DOI:
Determination of Quercitrin from Leaves by Capillary-zone Electrophoresis
Objective: To establish a new determination method for quercitrin from Platycladus orientalis leaves by capillary-zone electrophoresis (CZE). Method: Under the optimized CZE conditions:a running buffer composed of 30 mmol ·L-1 borax-boric acid (volume 1 ∶3)
separation voltage at 25 kV
temperature at 25 centigrade
injection time for 8 sec with 0.5 psi pressure
quercitrin from P. orientalis leaves was detected at 254 nm wavelength. Result: Quercitrin was linear in the range of 1-200 mg ·L-1 (r=0.999 3). The RSD of exactitude
stability
and recovery of the method was 1.1346%
2.6046 % and 2.3315%
respectively. The contents of quercitrin in three batches of P. orientalis leaves were 2.5513
2.5467
2.4780 mg ·g -1. Conclusion: The method was simple
fast and accurate for determination of the drugs containing quercitrin.