Determination of Quercetin Content in Heibai Baoganqing Granules of Miao Ethnomedicine by RP-HPLC

YANG Li-chang; YI Yin; LIN Jun-qing; ZHANG Xi-min

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Determination of Quercetin Content in Heibai Baoganqing Granules of Miao Ethnomedicine by RP-HPLC

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- Determination of Quercetin Content in Heibai Baoganqing Granules of Miao Ethnomedicine by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 11, Pages: 64-66(2010)
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- Published：2010
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YANG Li-chang, YI Yin, LIN Jun-qing, et al. Determination of Quercetin Content in Heibai Baoganqing Granules of Miao Ethnomedicine by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 64-66.
DOI：

YANG Li-chang, YI Yin, LIN Jun-qing, et al. Determination of Quercetin Content in Heibai Baoganqing Granules of Miao Ethnomedicine by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 64-66. DOI：

摘要

目的: 建立苗药黑白保肝清颗粒中槲皮素总量的测定方法。方法: 采用RP-HPLC法

Diamonsil(TM) 钻石 C18柱(4.6 mm×250 mm

5 μm)

以甲醇-0.2%磷酸(56 ∶44)为流动相

流速1.0 mL ·min-1

柱温35 ℃

检测波长370 nm。结果: 在0.020 6～0.133 μg进样量范围内

槲皮素含量与峰面积呈很好的线性关系( r=0.997)

平均回收率为98.2%

RSD为1.70%。5批样品中槲皮素总量均值为1.44 mg ·g-1。结论: 该方法简便

准确

重复性好

可用于苗药黑白保肝清颗粒中槲皮素总含量测定。

Abstract

Objective: To establish a method to determine quercetin in Heibai Baoganqing Granules of Mao ethnomedicine by HPLC. Method: HPLC was used. The column was Diamonsil (TM) C18 (4.6 mm×250 mm

5 μm). The mobile phase consisted of methanol-0.2% phosphoric acid (56 ∶44)

with the flow rate of 1.0 mL ·min-1 and the wavelength was at 370 nm. Result: Quercetin was linear in the range of 0.020 6～0.133 μg. The average recovery was 98.2%. The average content of quercetin in five samples was 1.44 mg ·g-1. Conclusion: This method is accurate

simple and reproducible

and can be used for the determination of quercetin in Heibai Baoganqing Granules.

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Related Author

YANG Li-chang

YI Yin

LIN Jun-qing

ZHANG Xi-min

ZHU Xiao-qin

PENG Shui-mei

WU Jin-zhong

HU Jing

Related Institution

School of Life Sciences,Guizhou Normal University

Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine

College of Pharmacy,Fujian University of Traditional Chinese Medicine

Tianjin University of Chinese Medicine

Xiyuan Hospital， China Academy of Chinese Medical Sciences

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