ObJective: To compare the influence of different alcohol extraction on the acute toxicity of Sophorae Tonkinensis Radix et Rhizome

caused by different extracted crafts. Method : Separately using percolate and reflux extracted methods to prepare the extracts of different processed products. The classical acute toxicity methods were used in this study

the acute toxical symptoms

signs

death time and accumulated death rate were recorded and the LD50were calculated by Bliss method. Result : The LD50 and its 95% confident limit of reflux and percolation extracts on mice were respectively 27.135(24.869-29.622)g ·kg-1 and 13.399(12.016-14.899)g ·kg-1

which are equal to 316.6 and 156.3 times of the daily dosage in clinical

the main acute toxical symptoms such as: hyperactivity

restlessness

intermittent convulsion

breathlessness and died of respiratory failure eventually. The body weight between survival and normal mice had obvious difference (P<0.05)

this difference disappeared after 7 d administration. Conclusion : The acute toxicity of different alcohol extraction of Sophorae Tonkinensis Radix et Rhizome is: percolation extracts>reflux extracts

explaining the key factors are temperature and concentration

especially the temperature

that affect the acute toxicity result expression of different alcohol extraction.