Determination of Ferulic Acid in Dandelion by HPCE

LI Xi-feng; QIU Tian-bao; HU Ya-nan; HAO Zhe; YU Yun-hui

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Determination of Ferulic Acid in Dandelion by HPCE

更新时间：2024-01-04

- Determination of Ferulic Acid in Dandelion by HPCE
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 16, Pages: 27-29(2010)
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- Published：2010
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LI Xi-feng, QIU Tian-bao, HU Ya-nan, et al. Determination of Ferulic Acid in Dandelion by HPCE[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 27-29.
DOI：

LI Xi-feng, QIU Tian-bao, HU Ya-nan, et al. Determination of Ferulic Acid in Dandelion by HPCE[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 27-29. DOI：

摘要

目的 :建立蒲公英中主要有效成分阿魏酸的高效毛细管电泳(HPCE)含量测定方法。方法 :采用高效毛细管电泳法

电泳条件:未涂层的弹性石英毛细管柱(75 μ m×57/50 cm)

电解质为20 mmol ·L-1硼砂溶液(pH 9.18)

分离电压18 kV

柱温25 ℃

压力进样5 s

检测波长328 nm。结果 :阿魏酸在0.002~0.012 g ·L-1呈良好线性关系

r=0.999 8

平均回收率为98.22%

RSD 1.78%。结论 :本法专属性强

简便快速

结果准确可靠

重复性好

适用于检测蒲公英中阿魏酸的含量。

Abstract

Objective: To establish a method for determining the contents of ferulic acid(FA) in dandelion by high-performance capillary electrophoresis (HPCE). Method: Dandelion was determined at 328nm using high-performance capillary electrophoresis (HPCE)

under the optimized conditions achieved with a elastic fused-sillia capillary tube(75 μm×57/50 cm)and 20 mmol ·L-1borate running buffer solution (pH 9.18) at a constant voltage of 18 kV and injecting pressure time of 5 s at 25 ℃. Result: FA was linear within the range of 0.002 to 0.012 g ·L-1 ( r =0.999 8). The average recovery 98.22%

RSD 1.78%. Conclusion: This method is simple

rapid

accurate and good repeatability in separation and determination of FA in Dandelion.

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Related Author

LI Xi-feng

DU Yun-feng

LV Fei-fei

HU Chang-jiang

WU Shan-shan

XIONG Rui

LI Xi-feng

HAO Zhe

Related Institution

Henan College of TCM

Jiangxi University of Chinese Medicine，Key Laboratory of Modern Preparation of Traditional Chinese Medicine，Ministry of Education

National Key Laboratory of Modern Chinese Medicine Creation of Classic Prescriptions

Laboratory Animal Science and Technology Center，Jiangxi University of Chinese Medicine

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

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