Determination of Euphol in Slices of  and Kongxian Pills by HPLC-ELSD

XIU Yan-feng; SHI Bei; ZHU Cheng

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Determination of Euphol in Slices of and Kongxian Pills by HPLC-ELSD

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- Determination of Euphol in Slices of and Kongxian Pills by HPLC-ELSD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 11, Pages: 61-63(2010)
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- Published：2010
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XIU Yan-feng, SHI Bei, ZHU Cheng. Determination of Euphol in Slices of and Kongxian Pills by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 61-63.
DOI：

XIU Yan-feng, SHI Bei, ZHU Cheng. Determination of Euphol in Slices of and Kongxian Pills by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 61-63. DOI：

摘要

目的: 建立甘遂及其制剂控涎丸中大戟二烯醇的含量测定方法。方法: 色谱柱为Dikma Diamonsil C18柱(4.6 mm×250 mm

5 μm)

流动相甲醇-水(99 ∶1)

流速1.0 mL ·min-1

检测器蒸发光散射检测器

柱温35 ℃。结果: 大戟二烯醇在进样量为1.04～20.80 μg( r=0.999 6)

进样量的常用对数值与峰面积的常用对数值呈良好的线性关系

平均回收率97.53%

RSD 2.56%

另外对10批甘遂饮片和5批控涎丸进行了测定。结论: 所建立的方法简单、准确、重复性好

可用于测定甘遂及其制剂控涎丸中大戟二烯醇的含量。

Abstract

Objective: To establish a HPLC method for the content determination of euphol in slices of E. kansui and its preparation Kongxian Pills. Method: HPLC-ELSD was carried out on a Dikma Diamonsil C18 column(4.6 mm×250 mm

5 μm)with methanol-water (99 ∶1) as the mobile phase

the flow rate was 1.0 mL ·min-1

and the detector was evaporative light-scattering detector(ELSD). The column temperature was set at 35 ℃. Result: The calibration curve was linear in the range of 1.04-20.80 μg ( r=0.999 6)

the average recovery was 97.53％with RSD of 2.56％. The content of euphol in ten batches of slices of E. kansui and five batches of Kongxian Pills were determined. Conclusion: The method is simple

accurate and reproducible

and it can be used to determine the content of euphol in slices of E. kansui and Kongxian Pills.

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Related Author

XIU Yan-feng1

SHI Bei1

ZHU Cheng1

ZHANG Xu-qian

XIAO Feng-xia

DENG Shao-dong

DENG Chao-ming

DENG Tao

Related Institution

College of Traditional Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine

Key Laboratory of Processing Traditional Chinese Material Media Nanjing University of Traditional Chinese Medicine

Third Affiliated Hospital of Henan University of Chinese Medicine

Luohe Medical College

Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine（TCM），School of Pharmacy，Henan University of Chinese Medicine

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