Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats

LAI Hai-biao; LIU Zhao-hui; WU Song; OU Xiu-hua; GU Xiang-ming; WANG Shu-qin; GAO Yu-qiao; LIN Hui; WANG Yun; DAI Wei-bo; FAN Wen-chang; QIU Lan-ying; LIN Jia-le; MEI Quan-xi

您当前的位置：

首页 >

文章列表页 >

Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats

更新时间：2024-09-23

- Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 14, Pages: 135-138(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2010
- 稿件说明：
移动端阅览
LAI Hai-biao, LIU Zhao-hui, WU Song, et al. Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 135-138.
DOI：

LAI Hai-biao, LIU Zhao-hui, WU Song, et al. Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 135-138. DOI：

摘要

目的: 筛选一种方法简便、稳定、成石效果好的实验性大鼠肾草酸钙结石造模方法。方法: 采用几种目前常用的实验性大鼠肾草酸钙结石造模方法(造模Ⅰ组:1%乙二醇饮水+2%氯化铵每天ig 2 mL/只;造模Ⅱ组:1.25%乙二醇+1%氯化铵

ig 20 mL ·kg-1 ·d-1;造模Ⅲ组:每只每天饮用1.25%乙二醇+1%氯化铵10 mL

水30 mL;造模Ⅳ组:1%乙二醇+1%氯化铵自由饮用)进行造模

并进行各组尿液、血液和肾组织中的多项生化指标【尿素氮(BUN)、肌酐(Cr)、尿酸(UA)、无机磷(IP)、钙(Ca2+)、草酸(OX)】检测以及肾脏病理标本观察

综合评价造模效果。结果: 造模Ⅰ组的24 h尿液中的BUN

Ca2+

OX均高于正常对照组(Ⅴ组)

且统计学上有极显著性差异(P<0.01);血液中BUN

Ca2+均高于正常对照组

且统计学上有极显著性差异(P<0.01);肾组织中Ca2+和OX均高于正常对照组

且统计学上有极显著性差异(P<0.01);肾组织可见大量草酸钙结晶沉积。造模Ⅱ组和造模Ⅲ组的多项生化指标结果显示大鼠的肾功能下降

但肾组织中未见结晶体

仅发现肾间质血管充血。造模Ⅳ组大鼠全部死亡。结论: 造模Ⅰ组为最佳造模方法。

Abstract

Objective: To select a simple

stable and satisfied experimental model of renal calcium oxalate calculus in rats. Method: After several renal calcium oxalate calculus models currently used (Model group I: administered intragastrically with 1% ethylene glycol free drink+2% ammonium chloride 2 mL/rat per day; model Group Ⅱ: administered intragastrically with 1.25% ethylene glycol+1% ammonium chloride 20 mL ·kg-1 per day; model group Ⅲ: drank 10 mL 1.25% ethylene glycol+1% ammonium chloride and 30 mL water per day; model group Ⅳ: drank 1% ethylene glycol+1% ammonium chloride freely.) experimental rat models were developed. Several biochemical indicators of urine

blood and nephridial tissue were measured and renal pathological specimens were observed. Then the modeling results were comprehensive evaluated. Result: The 24 h urinary BUN

Ca2+

OX of model groupⅠwere singnificantly higher than the normal control group (groupⅤ) (P<0.01); blood BUN

Ca2+ were significantly higher than the normal control group (P<0.01); nephridial tissue Ca2+ and OX were significantly higher than the normal control group (P<0.01); and calcium oxalate crystals were distributed in nephridial tissue. The several biochemical indicators of model group Ⅱ and Model group Ⅲ disclosed the rats renal function breakdown

and renal pathological specimens only displayed vascular engorgement in renal interstitium and calcium oxalate crystals hadnt been seen in nephridial tissue. All rats of model group Ⅳ were dead. Conclusion: Model groupⅠrepresents the best modeling for renal calculus of calcium oxalate.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats

Prevention of Extract from Rhizoma Phragmitis on Calcium Oxalate Stones in Male Rats

Analysis of Animal Models with Combined Allergic Rhinitis and Asthma Syndrome in Children Based on Clinical Disease-syndrome Characteristics of Traditional Chinese and Western Medicine

Establishment and Evaluation of Rat Model of Myocardial Ischemia-reperfusion Injury with Phlegm and Blood Stasis Blocking Collaterals Syndrome Based on Metabolomics

Establishment and Evaluation of Rat Model of Acute Myocardial Infarction in Coronary Heart Disease with Qi and Yin Deficiency Syndrome Based on Sleep Deprivation Combined with Coronary Artery Ligation

Related Author

LAI Hai-biao1

LIU Zhao-hui1

WU Song1

OU Xiu-hua2

GU Xiang-ming1

WANG Shu-qin1

GAO Yu-qiao1

LIN Hui1

Related Institution

Hospital of Traditional Chinese Medicine of Zhongshan

Guangzhou University of Traditional Chinese Medicine

Dongfang Hospital， Beijing University of Chinese Medicine

Yunnan Provincial Hospital of Traditional Chinese Medicine

School of Clinical Medicine，Beijing University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰