Determination of Content and Entrapment Efficiency of Vinorelbine Encapsulated in Liposomes
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Determination of Content and Entrapment Efficiency of Vinorelbine Encapsulated in Liposomes
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 11, Pages: 50-53(2010)
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Published:2010
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LI Xin, CHENG Lan, LI Xue-tao. Determination of Content and Entrapment Efficiency of Vinorelbine Encapsulated in Liposomes[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 50-53.
DOI:
LI Xin, CHENG Lan, LI Xue-tao. Determination of Content and Entrapment Efficiency of Vinorelbine Encapsulated in Liposomes[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(11): 50-53.DOI:
Determination of Content and Entrapment Efficiency of Vinorelbine Encapsulated in Liposomes
Objective: To establish a method to determine the content and entrapment efficiency of vinorelbine encapsulated in liposomes. Method: The HPLC analysis was carried out on a ELITE C18 (4.6 mm×250 mm
5 μm) column with mobile phase consisted of acetonitrile- 0.06 mol ·L-1 KDP buffer (38 ∶62) at a flow rate of 1.0 mL ·min-1.The detection wavelength was at 215 nm and the column temperature was 30 ℃.The free drug was isolated by Gel-filtration for the entrapment efficiency determination. Result: The 1inear range of vinorelbine was 2.08~20.8 μg (r=0.999 9).The average recovery was 98.64% (RSD 1.09%). The average entrapment efficiency was 86.83%. Conclusion: This method is simple
precise and replicable and it can be used to determine the content of total vinorelbine in liposomes and entrapment efficiency.
Determination of Content and Entrapment Efficiency of Vinorelbine Encapsulated in Liposomes
Preparation and Safety Evaluation of Vinorelbine Liposems
Preparation and its release in vitro of Vinorelbine encapsulated in liposomes
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Related Author
LI Xin
CHENG Lan
LI Xue-tao1
CHENG Lan
LI Xue-tao
TANG Ling
lixuetao
chenglan
Related Institution
The Medicine Institute of Liaoning University of Traditional Chinese Medicine
Third Affiliated Hospital of Henan University of Chinese Medicine
Luohe Medical College
Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine(TCM),School of Pharmacy,Henan University of Chinese Medicine
School of Pharmacy, Jiangxi University of Chinese Medicine