Determination of Cholic acid in Tibetan Medicine Preparation Ganxianxiao Granules by HPLC-ELSD

XU Meng-dan; JU Jian-ming; HUANG Yi-ping; WAN Yuan-song

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Determination of Cholic acid in Tibetan Medicine Preparation Ganxianxiao Granules by HPLC-ELSD

更新时间：2024-01-04

- Determination of Cholic acid in Tibetan Medicine Preparation Ganxianxiao Granules by HPLC-ELSD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 13, Pages: 39-40(2010)
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- Published：2010
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XU Meng-dan, JU Jian-ming, HUANG Yi-ping, et al. Determination of Cholic acid in Tibetan Medicine Preparation Ganxianxiao Granules by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(13): 39-40.
DOI：

XU Meng-dan, JU Jian-ming, HUANG Yi-ping, et al. Determination of Cholic acid in Tibetan Medicine Preparation Ganxianxiao Granules by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(13): 39-40. DOI：

摘要

目的 :测定复方藏药制剂肝纤消颗粒中胆酸的含量。方法 :采用HPLC-ELSD法进行含量测定

色谱条件为色谱柱AlltimaC18 (4.6 mm×250 mm

5 μm);流动相乙腈-0.3%乙酸溶液(40 ∶60);流速1.0 mL ·min-1;柱温35 ℃;ELSD漂移管温度100 ℃;载气流量2.6 L ·min-1;进样量20 μL

以外标两点对数方程计算样品的含量。结果 :胆酸的线性范围为2.052~32.832 μg

r =0.999 2

肝纤消颗粒样品中胆酸高、中、低浓度的加样回收率分别为97.1%

99.4%

97.4%

RSD分别为0.7%

0.4%

1.1%( n ＝3)。结论 :该方法简便、快速、准确、重复性好

可用于控制肝纤消颗粒的质量。

Abstract

Objective: To establish a method for the determination of cholic acid in the compound preparation Ganxianxiao Granules. Method: HPLC-ELSD was used in the quantitative analysis with the condition as follows. Alltima C18 (4.6 mm×250 mm

5 μm) was used as column; mobile phase was acetonitrile-0.3%HAc (40∶60); the temperature of column was at 35 ℃; flow rate was 1.0 mL ·min-1

the temperature of evaporator tube was set at 100 ℃; carrier gas flow was 2.6 L ·min-1 and the injection volume was 20 μL

calculating the content with logarithmic equation of external standard. Result: Cholic acid was linear in the range of 2.052~32.832 μg (r=0.999 2). The average recovery of high

middle

low concentration in Ganxianxiao Granules was 97.1%

99.4%

97.4%

with RSD 0.7%

0.4%

1.1%(n＝3) respectively. Conclusion: This method was simple

reliable

accurate

and could be used to control the quality of the compound preparation Ganxianxiao Granules.

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