ObJective: To establish an HPLC method for separation and determination of rhein

emodin and chrysophanol in Dahuangtongqi oral liquid. Method: The chromatographic condition was as follows: Inertsil ODS-SP (4.6 mm×150 mm

5 μm) column was used with the mobile phase of methanol-0.1% phosphate acid (72 ∶28); the flow rate was 1.0 mL ·min-1

column temperature was at 25 ℃ and detection wavelength was at 440 nm. Result: Rhein

emodin and chrysophanol in Dahuangtongqi Oral Liquid were linear in the range of 4.2-50.4 (r=0.999 9)

3.9-46.8 (r=0.999 7)

4.2-50.4 mg ·L-1 (r=0.999 9) respectively. The average recovery of rhein

emodin and chrysophanol was 97.68%

97.36%

97.27%

respectively. Conclusion: This method was simple. The results were accurate and reproducible. It can be used in the quality control of Dahuangtongqi oral liquid.