Study on Processing Methods of Fructus Forsythiae

ZHANG Shu-rong; PEI Xiang-ping; LIANG Xue-wei; HUANG Xuan; HUANG Can-lin

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Study on Processing Methods of Fructus Forsythiae

更新时间：2024-09-23

- Study on Processing Methods of Fructus Forsythiae
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 18, Pages: 33-36(2010)
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- Published：2010
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ZHANG Shu-rong, PEI Xiang-ping, LIANG Xue-wei, et al. Study on Processing Methods of Fructus Forsythiae[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 33-36.
DOI：

ZHANG Shu-rong, PEI Xiang-ping, LIANG Xue-wei, et al. Study on Processing Methods of Fructus Forsythiae[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 33-36. DOI：

摘要

目的 :确定青翘的最佳炮制方法。方法 :采用HPLC直接测定

Alltima C18色谱柱(4.6 mm×250 mm

5 μm)

甲醇-水梯度洗脱

检测波长 280 nm

室温

流速 0.8 mL ·min-1。结果 :连翘酯苷的回归方程为 Y =959 631.703 1 X +250 467.648 4

r =0.999 4

线性范围1.5~15 μg;连翘苷的回归方程为 Y =883 203.205 8 X -9 047.738 3

r =0.999 4

线性范围0.145~1.45 μg;连翘酯苷和连翘苷的加样回收率分别为97.9 %

99.2 %

RSD分别为2.30%

2.27%( n =6)。青翘药材蒸制20~30 min

连翘酯苷和连翘苷的含量最高。结论 :该测定方法准确、可靠

重复性好

可用于连翘中连翘酯苷和连翘苷的含量测定;青翘的炮制以蒸制20~30 min为宜。

Abstract

Objective: To determine the optimal processing method for Fructus Forsythiae. Method: HPLC was applied on the determination with Alltima C18 column (4.6 mm×250 mm

5 μm) and methanol - water as mobile phase by a gradient elution program at a flow rate of 0.8 mL ·min-1. The detection wavelength was at 280 nm and column temperature was at room temperature. Result: The regression equation of Forsythiaside was Y =959 631.703 1 X +250 467.648 4

r =0.999 4

Linear range was 1.5-15 μg

The regression equation of phillyrin was Y =883 203.205 8 X -9 047.738 3

r =0.999 4

Linear range was 0.145-1.45 μg. The average recovery of Forsythiaside and Phillyrin was 97. 9% with RSD 2. 30% and 99. 2% with RSD 2. 27% (n= 6 ) respectively. Content of the Forsythiaside and Phillyrin was in the maximum yield with the processing methods of steaming for 20-30 min steaming. Conclusion: The method was accurate and repeatable

can be applied to the quality control of Fructus Forsythiae. The optimal processing method of Fructus Forsythiae was steaming for 20-30 min.

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Related Author

ZHANG Shu-rong

PEI Xiang-ping

LIANG Xue-wei

HUANG Xuan

HUANG Can-lin

LU Li

YANG Yichen

WANG Erhuan

Related Institution

Shanxi University of Traditional Chinese Medicine

State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs，National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences

Shaanxi University of International Trade&Commerce

School of Pharmacy，Hanzhong Vocational and Technical College

Shaanxi Buchang Pharmaceutical Co. Ltd.，Xiʹan

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