Application of a Cellulose-producing Endophytic  sp. G-6 on Extraction of Total Flavonoids from

ZHAO Yi-lu; XU Yun-feng; ZHOU Jian-qin; CHEN Shao-hua; WANG Jian-wen

您当前的位置：

首页 >

文章列表页 >

Application of a Cellulose-producing Endophytic sp. G-6 on Extraction of Total Flavonoids from

更新时间：2024-01-04

- Application of a Cellulose-producing Endophytic sp. G-6 on Extraction of Total Flavonoids from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 16, Pages: 13-17(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2010
- 稿件说明：
移动端阅览
ZHAO Yi-lu, XU Yun-feng, ZHOU Jian-qin, et al. Application of a Cellulose-producing Endophytic sp. G-6 on Extraction of Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 13-17.
DOI：

ZHAO Yi-lu, XU Yun-feng, ZHOU Jian-qin, et al. Application of a Cellulose-producing Endophytic sp. G-6 on Extraction of Total Flavonoids from [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 13-17. DOI：

摘要

目的 :利用筛选的高产纤维素酶内生青霉菌的发酵液辅助提取银杏黄酮。方法 :采用单因素分析和正交试验

以银杏总黄酮提取率为指标

对酶辅助提取中影响总黄酮提取效果的主要因素(如酶解时间

温度

酶料比等)进行研究。结果 :通过单因素试验和正交试验

得出纤维素酶发酵液酶解银杏叶的最佳条件为:发酵液以酶料比15 ∶1加入银杏干粉中

在pH 5.0、温度50 ℃下

酶解处理3 h

该条件下的银杏黄酮提取率达到了1.33%

比常规醇提法提高了18.75%

与纯酶辅助提取率相近。结论 :内生青霉菌发酵液辅助法是提取银杏总黄酮的有效方法。

Abstract

Objective: To study the application of the crude cellulase in the culture of endophytic Penicillium sp.G-6 to total flavonoids extraction from leaves of Ginkgo biloba. Method: The process was studied by single factor analysis and orthogonal design L9(34) with the extraction rate of total flavonoids as comprehensive index

and the effect of the ratio of dry G. biloba leaves to crude enzymatic extracts

temperature

time

and pH value on extraction rate were also studied. Result: The extraction rate of total flavonoids was 1.33%

increased by 18.75% than that in regular extraction process

under the condition that dry G. biloba leaves were incubated with crude enzymatic extracts with 15 ∶1 (pH 5.0) at 50 ℃ for 3 h. Conclusion: The results presented a practical method of cellulase-assistant extraction process on total flavonoids in leaves of Ginkgo biloba.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on the Extraction Process for Total Glucosides of Yupingfeng with Orthogonal Design

Study on the Technology of Extraction and Purification of Flavonoies from Herba Potentillae Chinensis

Progress in the Study on Extraction and Purification of Diterpenes Compounds

The extraction and determination the contents of astragaloside

Study on Extraction Process of Total Flavonoids from Dewaxed Propolis with Ethanol by Warm-immersion

Related Author

GAO Jian1

LI Jun2

MA Bin-ji3

CHENG Wen-ming2

JIN Yong2

LV Xiong-wen2

YAN Chen2

Related Institution

Affiliated Hospital of Guilin Medical College

Guilin Medical University

Key Laboratory of Modern Preparation of Traditional Chinese Medicine

School of Pharmacy,Nanchang University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰