Effect of Zhenwu Decoction on Renal Interstitial Fibrosis in Rats

QIU Mo-yan; JIANG Yue; ZHAO Zong-jiang; LI Bao-qing; GAO Jie-dong; SHEN Li-hong; YANG Mei-juan; CHEN Yong-de; GUO Jie; WU Wei

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Effect of Zhenwu Decoction on Renal Interstitial Fibrosis in Rats

更新时间：2024-01-04

- Effect of Zhenwu Decoction on Renal Interstitial Fibrosis in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 17, Pages: 177-180(2010)
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- Published：2010
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QIU Mo-yan, JIANG Yue, ZHAO Zong-jiang, et al. Effect of Zhenwu Decoction on Renal Interstitial Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 177-180.
DOI：

QIU Mo-yan, JIANG Yue, ZHAO Zong-jiang, et al. Effect of Zhenwu Decoction on Renal Interstitial Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 177-180. DOI：

摘要

目的 :观察真武汤对肾间质纤维化大鼠的作用

并通过观察真武汤对肾脏Ⅰ

Ⅲ型胶原纤维的影响

探讨真武汤抗肾间质纤维化的作用机制。方法 : 采用"单肾切除加腺嘌呤灌胃法"建立大鼠慢性肾衰竭模型。治疗组予真武汤9.8

2.45 g ·kg-1 ig

对照组予尿毒清溶液ig。给药4周后处死大鼠取血测肌酐(Cr)

尿素氮(BUN)

钙(Ca2+)、磷(P3+)

肾脏切片分别进行HE染色、PASM染色、Masson染色、Sirius Red染色、Mallory染色

光镜下观察大鼠肾组织的病理形态学改变

HE染色进行肾小管间质病变半定量分析

PASM染色、Masson染色、Sirius Red染色进行肾脏组织病理学图像分析。结果 :在肾间质纤维化面积、肾小球系膜积分吸光度等方面

真武汤高、低剂量组的作用优于模型组和尿毒清组

P<0.05。真武汤高、低剂量组在肾间质中Ⅰ

Ⅲ型胶原分布面积等方面的作用与模型组比

P<0.05。结论 :真武汤具有抗肾间质纤维化的作用

其作用途径可能与抑制Ⅰ

Ⅲ型胶原纤维增生有关。

Abstract

ObJective: Observing the protective effect of Zhenwu Decoction on renal interstitial fibrosis in rat

to explore its role of anti-renal fibrosis and Ⅰ

Ⅲ collagen fibers affected in renal fibrosis. Method :The rat model of chronic renal failure was used. Niaoduqing control group was treated by Niaoduqing solution

Zhenwu Decoction group was treated by Zhenwu Decoction concentrated solution

up to 28 days. After 4 weeks

blood samples were taken from the femoral artery. Serum creatinine

blood urea nitrogen

serum calcium and phosphorus were detected. Renal pathological staining for paraffin sections was carried out by hemotoxylin and eosin staining

periodic acid-silver methenamine (PASM) staining

Masson staining

Sirius red staining

Mallory staining. HE staining was randomly selected for semi-quantitative analysis in tubulointerstitial lesions. Periodic acid-silver methenamine staining

Masson staining

Sirius red staining

Mallory staining were randomly selected for kidney pathology analysis. Result :In PASM mesangial integral optical density quantitative model of mesangial proliferative

Masson staining fibrosis in the area of renal interstitial

Low-dosage Zhenwu Decoction and High-dosage Zhenwu Decoction group appeared to be better than model group and Niaoduqing control group (P<0.05). Low-dosage Zhenwu Decoction and High-dosage Zhenwu Decoction group appeared to be less than model group in renal interstitial of type Ⅰ and Ⅲ distribution area (P<0.05). Conclusion :Zhenwu Decoction shows effect of anti-renal interstitial fibrosis. And it inhibit Ⅰ

Ⅱ collagen fibers proliferation

which may be the mechanism for Zhenwu Decoction to treat renal interstitial fibrosis and chronic renal failure.

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