Purification Process of Total Flavonoids from with Macroporous Resins

ZENG Yong-chang; LIANG Shao-yu; XING Xue-feng; YU Li-ping; LUO Jia-bo

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Purification Process of Total Flavonoids from with Macroporous Resins

更新时间：2024-09-23

- Purification Process of Total Flavonoids from with Macroporous Resins
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 18, Pages: 26-28(2010)
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- Published：2010
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ZENG Yong-chang, LIANG Shao-yu, XING Xue-feng, et al. Purification Process of Total Flavonoids from with Macroporous Resins[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 26-28.
DOI：

ZENG Yong-chang, LIANG Shao-yu, XING Xue-feng, et al. Purification Process of Total Flavonoids from with Macroporous Resins[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 26-28. DOI：

摘要

目的 :为提高白花蛇舌草(HD)总黄酮的纯度

筛选适宜HD总黄酮纯化的大孔树脂

确定HD总黄酮纯化工艺参数。方法 :采用静态与动态吸附-解吸附方法对HD总黄酮进行纯化

以吸附率与洗脱率等指标优化工艺

利用紫外-可见分光光度计测量HD总黄酮的含量。结果 :HPD-826型大孔树脂纯化效果最好

其最佳工艺为上样流速2 BV ·h-1

上样液pH 5

上样浓度1.5 g ·L-1

洗脱剂pH 7

乙醇浓度80％与洗脱速度2 BV ·h-1;纯化后总黄酮含量高达91.24%。结论 :该工艺操作简单、稳定可行

重复性好

适合HD总黄酮纯化。

Abstract

Objective: Purify the total flavonoids from Hedyotis diffusa. with macroporous resin and establish optimum process for separating and purifying total flavonoids from H.diffusa. Method: The optimal techniques of separation and purification of flavonoid were obtained by static adsorption and elution tests

and the separation techniques were evaluated by measuring the concentration of total flavonoids from H.diffusa with ultraviolet spectrophotometer. Result : The HPD-826 macroporous resin gave the best effect of separation with the sampling rate 2 BV ·h-1

concentration 1.0 g ·L-1

pH 5 respectively

and the concentration of elution was 80％ alcohol

eluting velocity 2 BV ·h-1

with pH 7. Conclusion : HPD-826 resin could be applied to purify total flavonoids from H.diffusa.

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Related Author

ZENG Yong-chang

LIANG Shao-yu

YU Li-ping

LUO Jia-bo

罗佳波

XIAO Ting

YAN Zhi-yong

ZUO Chang-ying

Related Institution

Southen University Key Lab of Traditional Chinese Medicine and New Drug,Guangdong Provincial Key Lab of Traditional Chinese Medicine

Pharmacy Technology Center，Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

School of Life Sciences， Tsinghua University

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking Union Medical College

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