Determination and Comparison of Alkaloids Content between Cortex Phellodendri and Ermiao Wan Categorized Formulas by HPLC

SHEN Juan; YIN Lian; DUAN Jin-ao

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Determination and Comparison of Alkaloids Content between Cortex Phellodendri and Ermiao Wan Categorized Formulas by HPLC

更新时间：2024-01-04

- Determination and Comparison of Alkaloids Content between Cortex Phellodendri and Ermiao Wan Categorized Formulas by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 13, Pages: 31-34(2010)
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- Published：2010
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SHEN Juan, YIN Lian, DUAN Jin-ao. Determination and Comparison of Alkaloids Content between Cortex Phellodendri and Ermiao Wan Categorized Formulas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(13): 31-34.
DOI：

SHEN Juan, YIN Lian, DUAN Jin-ao. Determination and Comparison of Alkaloids Content between Cortex Phellodendri and Ermiao Wan Categorized Formulas by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(13): 31-34. DOI：

摘要

目的 :比较黄柏生物碱配伍二妙丸类方前后含量的变化

探讨类方方剂物质基础配伍变化的特征。方法 :采用HPLC

Kromasil C18色谱柱(4.6 mm ×250 mm

5 μm)

流动相为乙腈-0.1%磷酸溶液(50 ∶50

每100 mL加十二烷基磺酸钠0.17 g)

流速0.8 mL ·min-1

柱温30 ℃

检测波长345 nm。测定黄柏及其二妙丸类方(二妙方、三妙方、四妙方、加味四妙方)中小檗碱、巴马汀及药根碱成分含量。结果 :3种生物碱在四妙方中含量基本没变化

在二妙方、三妙方中含量减少

在加味四妙方中减少较多

药根碱较小檗碱、巴马汀在二妙丸类方中减少量大。结论 :黄柏配伍二妙丸类方后

黄柏生物碱成分在基本方二妙及不同类方中呈现一定的变化规律。

Abstract

Objective: To evaluate correlativity between the content of alkaloids in Cortex Phellodendri and Ermiao Wan categorized formulas. Method: The HPLC method was adopted to determine the content of berberine

palmatine and jatrorrhizine in Cortex Phellodendri and the categorized formulas of Ermiao Wan

Sanmiao Wan

Simiao Wan and Jiawei Simiao Wan. Chromatography parameters were A Kromasil C18 column (4.6 mm ×250 mm

5 μm) was used with the mobile phase of acetonitrile-0.1% phosphoric acid (50 ∶50) (adding SDS 0.17 g per 100 mL solution)

flow rate 0.8 mL·min-1

column temperature at 30 ℃

detection wavelength at 345 nm. Result: The content of three kinds of alkaloids was almost not changed in Simiao Wan

but decreased in Ermiao Wan and Sanmiao Wan

especially in Jiawei Simiao Wan. The content of jatrorrhizine was decreased much more than berberine and palmatine after compatibility. Conclusion: The alkaloids content presents a rule in Ermiao Wan categorized formulas after compatibility.

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Related Author

LIAN Lian

WAN Guo-sheng

JIA Li-wei

GAO Hui-yuan

CHEN Jian-long

ZHANG Yu-ling

DONG Yu

CAI Guang-zhi

Related Institution

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Shuguang Hospital Affiliated to Shanghai University of TCM

Shaanxi University of Chinese Medicine

The Affiliated Hospital of Shaanxi University of Chinese Medicine

Changzhou Hospital of Traditional Chinese Medicine（TCM）

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