Study on Curcumin in Decoction of Rhizoma Curcumae alone and that of Rhizoma Curcumae together with Rhizoma Sparganii

LI Lin; YIN Fang-zhou; LU Tu-lin; SHAO Yi; XU Kai-hui

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Study on Curcumin in Decoction of Rhizoma Curcumae alone and that of Rhizoma Curcumae together with Rhizoma Sparganii

更新时间：2024-01-04

- Study on Curcumin in Decoction of Rhizoma Curcumae alone and that of Rhizoma Curcumae together with Rhizoma Sparganii
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 13, Pages: 28-30(2010)
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- Published：2010
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LI Lin, YIN Fang-zhou, LU Tu-lin, et al. Study on Curcumin in Decoction of Rhizoma Curcumae alone and that of Rhizoma Curcumae together with Rhizoma Sparganii[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(13): 28-30.
DOI：

LI Lin, YIN Fang-zhou, LU Tu-lin, et al. Study on Curcumin in Decoction of Rhizoma Curcumae alone and that of Rhizoma Curcumae together with Rhizoma Sparganii[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(13): 28-30. DOI：

摘要

目的 :建立莪术中姜黄素含量测定的方法

并考察莪术与三棱配伍前后姜黄素煎出率的变化。方法 :采用HPLC法测定莪术药材、莪术单煎液、莪术三棱共煎液中姜黄素的含量

以Eclipse Plus C18色谱柱为分析柱

以水和乙腈为流动相进行梯度洗脱

检测波长425 nm

柱温25 ℃

流速1 mL ·min-1。结果 :姜黄素在进样量0.67~13.40 ng有良好线性关系( r=0.999 7)

平均回收率99.76%

RSD 1.49%

莪术和三棱配伍煎煮后

其姜黄素的煎出率比莪术单煎显著提高。结论 :所建立的姜黄素含量测定方法操作简便快速

结果准确可靠。两味中药在配伍煎煮后可有效改善姜黄素的煎出效果

推测在煎煮时三棱对姜黄素起到了保护作用。

Abstract

Objective: To establish a method for the content determination of curcumin in rhizoma curcumae

and compare the outcome between decoction of curcumin in rhizoma curcumae alone and that of rhizoma curcumae together with rhizoma sparganii. Method: HPLC was performed on a eclipse plus C18 column

water- acetonitrile served as mobile phase and the detection wavelength was at 425 nm

the column temperature at 25 ℃ and the flow rate being 1.0 mL ·min-1

to determine the content of curcumin in different decoction. Result: Curcumin showed good linearity in the range of 0.67-13.40 ng with correlation coefficient of 0.999 7. The average recovery (n=9) was 99.76%

with RSD of 1.49%. The content of curcumin in the co-decoction of the two drugs was significantly higher than that in the decoction of rhizoma curcumae alone. Conclusion: The method used was handy

sensitive and accurate

and proves to be suitable for the content determination of Curcumin.and it is suggest that rhizoma sparganii play a protective effect on the curcumin when the two drugs being decocted.

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