Radical Scavenging Activity of

SHE Gai-mei; SUN Fang-fang; LV Hai-ning; LIU Bin

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Radical Scavenging Activity of

更新时间：2024-09-23

- Radical Scavenging Activity of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 18, Pages: 91-94(2010)
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- Published：2010
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SHE Gai-mei, SUN Fang-fang, LV Hai-ning, et al. Radical Scavenging Activity of [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 91-94.
DOI：

SHE Gai-mei, SUN Fang-fang, LV Hai-ning, et al. Radical Scavenging Activity of [J]. Chinese journal of experimental traditional medical formulae, 2010, 16(18): 91-94. DOI：

摘要

目的 :研究多叶棘豆的清除自由基活性。方法 : 分别以常用抗氧化剂L-抗坏血酸和生育酚为对照

采用清除DPPH和ABTS自由基的方法对多叶棘豆乙醇提取物不同极性部位

以及AB-8大孔吸附树脂柱不同乙醇浓度洗脱部位进行清除自由基能力的评价。结果 : 多叶棘豆提取物AB-8大孔吸附树脂柱的50%乙醇洗脱部位对DPPH自由基和ABTS自由基均有较强的清除能力

其清除DPPH自由基能力强于同浓度的L-抗坏血酸。结论 : AB-8大孔吸附树脂柱层析技术对多叶棘豆的清除自由基活性物质有分离富集作用

以50%乙醇洗脱部位活性最为突出。

Abstract

Objective: To study DPPH and ABTS radical scavenging activities of Oxtropis myriophylla. Method: The anti-oxidant activity of O . myriophylla was measured by DPPH assay and ABTS assay. Furthermore

the AB-8 macroporous absorbent resincolumn chromatography with gradient elution (aqueous solution

50% and 90% ethanol solution)were used. Result: 50% ethanol extract from O . myriophylla in the capability of scavenging DPPH and ABTS free radicals was much stronger than any other fractions

which is a trifle stronger than ascorbic acid. In addition

the active ingredient was screened effectively by the column chromatography method. Conclusion: 50% ethanol extract from O . myriophylla in the capability of scavenging DPPH and ABTS free radicals was much stronger than any other fractions

which is a trifle stronger than ascorbic acid. In addition

the active ingredient was screened effectively by the column chromatography method. Conclusion: The 50% ethanol extracts exhibited strong scavenging capacity of free radical.

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Related Author

LIU Bin

LV Hai-ning

SHE Gai-mei

SUN Fang-fang

MIAO Jinxin

MIAO Mingsan

WANG Jun

WU Siqing

Related Institution

School of Chinese Pharmacy,Beijing University of Chinese Medicine

Henan University of Chinese Medicine

Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao， Henan University of Chinese Medicine

Guangxi University of Chinese Medicine

Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine

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