Effect of Ash Bark from Different Origins and Coumarin Monomer on Inflammation Factors Released from RAW 264.7 Cells Induced by Lipopolysaccharide

Yang Qing; WENG Xiao-gang; NIE Shu-qin; SUN Jian-hui; LIU Li-mei; WANG Rui-hai; CHEN Lin

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Effect of Ash Bark from Different Origins and Coumarin Monomer on Inflammation Factors Released from RAW 264.7 Cells Induced by Lipopolysaccharide

更新时间：2024-01-04

- Effect of Ash Bark from Different Origins and Coumarin Monomer on Inflammation Factors Released from RAW 264.7 Cells Induced by Lipopolysaccharide
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 13, Pages: 127-131(2010)
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- Published：2010
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Yang Qing, WENG Xiao-gang, NIE Shu-qin, et al. Effect of Ash Bark from Different Origins and Coumarin Monomer on Inflammation Factors Released from RAW 264.7 Cells Induced by Lipopolysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(13): 127-131.
DOI：

Yang Qing, WENG Xiao-gang, NIE Shu-qin, et al. Effect of Ash Bark from Different Origins and Coumarin Monomer on Inflammation Factors Released from RAW 264.7 Cells Induced by Lipopolysaccharide[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(13): 127-131. DOI：

摘要

目的 :研究4种秦皮提取物、5种香豆素单体、5种单体混合物、苦枥白蜡树已知香豆素指纹区样品和未知成分指纹区样品对内毒素(LPS)刺激小鼠单核-巨噬细胞株RAW264.7细胞分泌炎症因子的影响。方法 :以 LPS 10 mg·L-1刺激RAW264.7细胞

加入不同浓度的待测样品

24h后

取培养液上清测定肿瘤坏死因子-α(TNF-α)及白介素-1α(IL-1α)含量。结果 :①4种基原秦皮提取物能显著抑制由LPS诱导巨噬细胞分泌TNF-α(P<0.05)。②5种香豆素单体均能显著抑制由LPS诱导巨噬细胞分泌IL-1α;除秦皮苷外其余4种单体

能显著抑制由LPS诱导巨噬细胞分泌TNF-α

其中秦皮甲素、秦皮素具有量效关系。③苦枥白蜡树提取物

5种香豆素单体混合物

苦枥白蜡树已知香豆素指纹区样品和未知成分指纹区样品能显著抑制由LPS诱导巨噬细胞分泌TNF-α(P<0.05)

5种香豆素单体混合物能显著抑制LPS诱导巨噬细胞产生IL-1α

其他样品的作用没有显著性。结论 :在该测试系统中4种基原秦皮中尖叶白蜡树抗炎活性最强

其他3个品种抗炎活性相近

抗炎作用与素皮已知香豆素含量没有直接的量效关系

说明素皮除已知香豆素外

未知成分对炎症因子也有一定抑制作用;5种香豆素单体中秦皮乙素

7-二甲氧基-8-羟基香豆素抗炎活性最强。

Abstract

Objective: To investigate the anti-inflammatory effects for a number of samples on RAW264 cell. The samples include: four kinds of origins of ash bark extract

five kinds of coumarin monomer

five kinds of monomer mixture

known coumarin fingerprint region sample and unknown component fingerprint region from Fraxinus rhynchophylla Hance. Method: RAW264.7 cell line was treated by 10 mg ·L-1 lipopolysaccharide (LPS)

24 hours later

the supernatant was collected and the inflammatory factors such as TNF-α and IL-1α were measured. Result: Four kinds of origins ash bark extract could significantly inhibited the LPS-induced macrophages to produce TNF-α (P<0.05). Five kinds of coumarin monomer could significantly inhibit the LPS-induced macrophages produced IL-1α. Except fraxin four kinds of monomers could significantly inhibit the LPS-induced macrophages to produce TNF-α

including aesculin and fraxetol with dose-effect relationship. Only five kinds of coumarin monomer mixtures could significantly inhibit the LPS-induced macrophages produced levels of IL-1α. Other samples was not significant. Conclusion: F. chinensis Roxb.var.acuminata Lingelsh. shows most powerful anti-inflammatory activity among 4 kinds of origins ash bark extract. The other 3 kinds of Origins ash bark extract is similar for anti-inflammatory activity. Aesculetion and 6

7-dichomethoxyl-8-hydroxycoumarin show most powerful anti-inflammatory activity in 5 kinds of coumarin monomer.

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