Jianpi Jiedu Recipe Intervenes Signal Transduction of p38 Mitogen-activated Protein kinases and Inhibits Activity of Cyclooxygenase-2 Promoter Induced by  in Gastric Cancer Cell MKN45

WANG Yan; LIU Ning-ning; ZHOU Li-hong; WU Qiong; ZHOU Ning; SUN Yu; FAN Zhong-ze; LI Qi

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Jianpi Jiedu Recipe Intervenes Signal Transduction of p38 Mitogen-activated Protein kinases and Inhibits Activity of Cyclooxygenase-2 Promoter Induced by in Gastric Cancer Cell MKN45

更新时间：2024-09-23

- Jianpi Jiedu Recipe Intervenes Signal Transduction of p38 Mitogen-activated Protein kinases and Inhibits Activity of Cyclooxygenase-2 Promoter Induced by in Gastric Cancer Cell MKN45
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 14, Pages: 105-109(2010)
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WANG Yan, LIU Ning-ning, ZHOU Li-hong, et al. Jianpi Jiedu Recipe Intervenes Signal Transduction of p38 Mitogen-activated Protein kinases and Inhibits Activity of Cyclooxygenase-2 Promoter Induced by in Gastric Cancer Cell MKN45[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 105-109.
DOI：

WANG Yan, LIU Ning-ning, ZHOU Li-hong, et al. Jianpi Jiedu Recipe Intervenes Signal Transduction of p38 Mitogen-activated Protein kinases and Inhibits Activity of Cyclooxygenase-2 Promoter Induced by in Gastric Cancer Cell MKN45[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 105-109. DOI：

摘要

目的: 探讨幽门螺杆菌(Helicobacter pylori)对人胃癌MKN45细胞环氧合酶2(Cyclooxygenase-2

COX-2)启动子荧光素酶报告基因重组质粒(pGL3-Basic-COX-2-promoter)活性的影响和健脾解毒方对其的调控作用及可能的机制。方法: 将构建的pGL3-Basic-COX-2-promoter转染MKN45细胞

观察H.pylori对其活性的影响。运用p38MAPK特异性抑制剂SB203580阻断p38MAPK信号通路

观察H. pylori对MKN45细胞COX-2启动子活性的影响。Western blot法检测健脾解毒方对H. pylori感染的MKN45细胞p38MAPK信号通路及其下游激活转录因子-2(ATF-2)表达的影响。结果: H.pylori可增加COX-2启动子活性;抑制p38MAPK信号通路后

COX-2启动子活性明显下调;健脾解毒方能够抑制H. pylori诱导的p38MAPK及ATF-)的活性。结论: H. pylori通过p38MAPK信号通路上调胃癌细胞COX-2启动子活性;健脾解毒方通过调控p38MAPK/ATF-2信号通路

抑制COX-2启动子活性

是其防治H. pylori诱发胃癌的机制之一。

Abstract

Objective: To investigate the effect of Helicobacter pylori on pGL3-Basic-COX-2-promoter transcriptional activity in gastric cancer MKN45 cells and the regulatory mechanism of Jianpi Jiedu Recipe on COX-2 promoter transcriptional activity. Method: The recombinant vector pGL3-Basic-COX-2-promoter was transient co-transfected into MKN45 cells

then detected the effect of H. pylori on COX-2 promoter activity. The effect of H. pylori on COX-2 promoter activity in human gastric cancer cells after blocking p38MAPK signal transduction pathway with a specific inhibitor SB203580 was observed. The effect of Jianpi Jiedu Recipe on H. pylori-stimulated phosphorylation of p38MAPK and ATF-2

the downstream transcription factor of p38MAPK

was investigated. Result: H.pylori could increase COX-2 promoter activity. COX-2 promoter activity down-regulated significantly after blocking p38MAPK signal transduction pathway. Jianpi Jiedu Recipe inhibited H. pylori-induced p38MAPK and ATF-2 activity. Conclusion: H. pylori infection increases COX-2 promoter transcriptional activity via p38MAPK signal transduction pathway

and Jianpi Jiedu Recipe inhibits H. pylori-induced COX-2 promoter transcriptional activity through regulating p38MAPK/ATF-2 signal transduction pathway

which may be one of the mechanisms of prevention and treatment of gastric cancer.

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Related Author

WANG Yan1

LIU Ning-ning1

ZHOU Li-hong1

WU Qiong1

ZHOU Ning1

SUN Yu2

FAN Zhong-ze2

LI Qi1

Related Institution

Laboratory Center,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Oncology,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Graduate School， Hebei University of Chinese Medicine

Hebei Provincial Hospital of Traditional Chinese Medicine

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