Determination of Rutin and Quercetin in Tartary Buckwheat Extract
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Determination of Rutin and Quercetin in Tartary Buckwheat Extract
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 17, Pages: 60-62(2010)
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Published:2010
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ZOU Liang, WANG Zhan-guo, HU Hui-ling, et al. Determination of Rutin and Quercetin in Tartary Buckwheat Extract[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 60-62.
DOI:
ZOU Liang, WANG Zhan-guo, HU Hui-ling, et al. Determination of Rutin and Quercetin in Tartary Buckwheat Extract[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 60-62.DOI:
Determination of Rutin and Quercetin in Tartary Buckwheat Extract
ObJective: To determine the content of rutin and quercetin in the tartary buckwheat extract
so as to appraise its quality. Method: The chromatographic conditions were as follows: with the Welchrom-C18 (4.6 mm×150 mm
5 μm) as the chromatographic column
methanol-0.2% formic acid solution (50:50) as the mobile phase
280 nm as the detection wavelength
30 ℃ as the column temperature
1.0 mL ·min-1 as the flow rate. Result: The linear range for rutin was 1.56-100.00 mg[DK]·L-1 and the linear range for quercetin was 1.52-97.00 mg[DK]·L-1. The average recovery for rutin and quercetin was 98.49% (RSD 1.25%
n=5) and 97.87% (RSD 1.69%
n=5) respectively. Conclusion: Rutin and quercetin were 18.97%±1.43% and 5.03%±0.42% respectively determined by the method applied.