Studies on Difference between Analgesic Effects and Chemical Compositions of Danggui Shaoyao San Extracted by Water and Ethanol
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Studies on Difference between Analgesic Effects and Chemical Compositions of Danggui Shaoyao San Extracted by Water and Ethanol
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 16, Pages: 89-93(2010)
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Published:2010
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CAO Dan-hua, GAO Zhen, KOU Jun-ping, et al. Studies on Difference between Analgesic Effects and Chemical Compositions of Danggui Shaoyao San Extracted by Water and Ethanol[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 89-93.
DOI:
CAO Dan-hua, GAO Zhen, KOU Jun-ping, et al. Studies on Difference between Analgesic Effects and Chemical Compositions of Danggui Shaoyao San Extracted by Water and Ethanol[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 89-93.DOI:
Studies on Difference between Analgesic Effects and Chemical Compositions of Danggui Shaoyao San Extracted by Water and Ethanol
Objective: To compare the analgesic effects and chemical components for two kinds of extracts of Danggui Shaoyao San (DSS) by water and 50% ethanol accordingly
so as to provide some evidence for its clinical use and exploration of its material basis. Method: Two kinds of extracts of DSS were prepared by water reflux and ultrasonic wave-assisted extraction by 50% ethanol respectively
and their analgesic activities were evaluated in acetic acid-induced wringing and oxytocin-induced menorrhalgia in mice.Meanwhile
chemical constituents and total sugar content of two kinds of extracts were analyzed by high-performance liquid chromatography with Diode Array Detector (HPLC-DAD) and phenol-sulfuric acid method
respectively. Result: Water reflux extract of DSS (2.5 g ·kg-1
10 g ·kg-1) and ethanol extract of DSS (10 g ·kg-1) significantly decreased writhing times induced by acetic acid and oxytocin
while the inhibition rate of water extract was higher than that of ethanol extraction of DSS.Meanwhile
about 20 fingerprint peaks were found in ethanol extract of DSS.However
the chromatographic peak area of 4 compounds possibly come from Paenonia lactiflora Pall and total sugar contents in water extract of DSS were higher than that of ethanol extract. Conclusion: The analgesic effect of water reflux extraction of DSS was better than that of ultrasonic wave-assisted extraction by 50% ethanol
which might be partly due to the higher contents of the polar compositions and polysaccharides.
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