Objective: To investigate the chemical constituents of Celastrus vamotii. Method: The air-dried roots and stems of C.vamotii were powdered and refluxed with 95% EtOH for three times. The combined extract were evaporation under reduced pressure to give residue

which was suspended in water and fractionated with ethyl acetate. Result : Five compounds were isolated from the EtOAc fraction and identified on basis of spectral and chemical evidence such as MS

1H-NMR

and 13C-NMR etc. They are β-sitosterol (1)

β-amyrin (2)

β-amyrin acetate (3)

30-hydroxylup-20 (29)-en-3-one (4)

and 3-oxolup-20-(29)-en-30-al (5). Conclusion: All compounds were isolated from the plant for the first time.