Influence of Drug-Containing Serum of Yiqi Fuyuan Tang Combined with Chemotherapy on Bcl-2, Bax Expression of Colorectal Cancer Lovo Cell

QIN Wei-fu; HE Ju-qiao

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Influence of Drug-Containing Serum of Yiqi Fuyuan Tang Combined with Chemotherapy on Bcl-2, Bax Expression of Colorectal Cancer Lovo Cell

更新时间：2024-01-04

- Influence of Drug-Containing Serum of Yiqi Fuyuan Tang Combined with Chemotherapy on Bcl-2, Bax Expression of Colorectal Cancer Lovo Cell
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 17, Pages: 158-161(2010)
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- Published：2010
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QIN Wei-fu, HE Ju-qiao. Influence of Drug-Containing Serum of Yiqi Fuyuan Tang Combined with Chemotherapy on Bcl-2, Bax Expression of Colorectal Cancer Lovo Cell[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 158-161.
DOI：

QIN Wei-fu, HE Ju-qiao. Influence of Drug-Containing Serum of Yiqi Fuyuan Tang Combined with Chemotherapy on Bcl-2, Bax Expression of Colorectal Cancer Lovo Cell[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 158-161. DOI：

摘要

目的: 观察益气复元汤是否增加化疗药诱导大肠癌Lovo细胞凋亡的影响以及其分子水平作用机制。方法: 试验分为益气复原汤、化疗药、益气复原汤+化疗药各自含药血清、血清对照组、空白对照组等5组

人吖啶橙/溴乙啶(AO/EB)双荧光染色法、Annexin V-FITC(FITC

异硫氰酸荧光素)单染法检测各组对大肠癌Lovo细胞凋亡诱导作用

Western blot(蛋白免疫印迹)法检测Lovo细胞凋亡蛋白B细胞淋巴瘤/白血病-2(Bcl-2)

多肽抗原(Bax)的表达。结果: 细胞形态学及流式细胞检测都表明益气复原汤含药血清对大肠癌Lovo细胞有诱导凋亡作用

并增强化疗药诱导癌细胞凋亡效应;Western blot法检测显示①血清对照组与空白对照组中Bcl-2

Bax蛋白表达无明显差异;②益气复元汤联合化疗组、化疗组、益气复元汤组、血清对照组各组Bcl-2

Bax蛋白表达组间两两比较具有显著差异

P<0.01;益气复元汤联合化疗组Bcl-2表达最弱

其次是化疗组

再次是益气复元汤组

血清对照组最强。③益气复元汤联合化疗组Bax表达最强

其次是化疗组

再次是益气复元汤组

血清对照组最弱。结论: 益气复元汤对Lovo肠癌细胞有诱导细胞凋亡作用

并增强化疗药诱导癌细胞凋亡效应;下调Bcl-2蛋白及上调Bax蛋白的表达是诱导Lovo肠癌细胞凋亡的主要机制之一。

Abstract

ObJective: To observe influence of Yiqi Fuyuan Tang(YQFYT) combined with chemotherapy on apoptosis of colorectal cancer Lovo cells and the related molecular mechanism. Method: Experiment was divided into 5 groups: YQFYT-containing serum group

chemotherapeutic drugs-containing serum group

YQFYT+chemotherapeutic drugs-containing serum group

serum control group and blank control group. Apoptosis of colorectal cancer Lovo cells was investigated with AO/EB double fluorescent staining and single staining of Annexin V-FITC for each group. Expression of apoptosis proteins Bcl-2 and Bax of Lovo cell was determined. Result: The results indicated that YQFYT-containing serum had action of inducing apoptosis in colorectal cancer Lovo cells

and enhanced the effects of induced apoptosis of chemotherapeutic drugs. Western blot assay showed that:①Bcl-2

Bax protein expression was not significantly different between serum control group and blank control group; ②There was significant difference between treatment groups (P<0.01). The order from weak to strong of the Bcl-2 protein expression was as follows: YQFYT+ chemotherapeutic drugs-containing serum group

chemotherapeutic drugs-containing serum group

YQFYT-containing serum group

serum control group. The order for bax protein expression was Just the opposite. Conclusion: Serum YQFYT-containing serum has action of inducing apoptosis in colorectal cancer Lovo cells

and can enhance the effects of chemotherapeutic drugs for inducing apoptosis. Down-regulation of Bcl-2 protein expression and up-regulation of Bax protein expression might be one of the main mechanisms for apoptosis of Lovo colon cancer cells.

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