Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 17, Pages: 53-56(2010)
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Published:2010
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LI Wei-dong, ZHANG Zhi-Jie, CAI Bao-chang. Research on HPCE Fingerprint of Tongsaimai Pellet[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 53-56.
DOI:
LI Wei-dong, ZHANG Zhi-Jie, CAI Bao-chang. Research on HPCE Fingerprint of Tongsaimai Pellet[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 53-56.DOI:
ObJective: To establish an HPCE method for determining the fingerprint of Tongsaimai pellet. Method :The buffer was composed of 40 mmol ·L-1borax (pH 9.18)-methanol (90 ∶10).CE was performed on a 75 μm×56 cm (effective length is 50.0 cm)fused silica capillary.Separation voltage was 25 kV
sampling pressure was 5 kPa
sampling time was 2.0 s
the temperature was maintained at 25 ℃
and detected at 254 nm. Result :Based on the fingerprints of 10 batches Tongsaimai pellet
an average electropherogram was used as the standard fingerprint
among which there were 16 characteristic peaks. Conclusion :The fingerprint analysis can be used for assessing the quality consistency of Tongsaimai pellet from batch to batch.