Simultaneous Determination of Gastrodine and Guanosine in Banxia Baizhu Tianmatang

WANG Xun; LU Jia-feng; LUO Shan-shan; WANG Xian-zhen; JIANG Jia-ye; KE Yan

您当前的位置：

首页 >

文章列表页 >

Simultaneous Determination of Gastrodine and Guanosine in Banxia Baizhu Tianmatang

更新时间：2024-01-04

- Simultaneous Determination of Gastrodine and Guanosine in Banxia Baizhu Tianmatang
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 17, Pages: 44-47(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2010
- 稿件说明：
移动端阅览
WANG Xun, LU Jia-feng, LUO Shan-shan, et al. Simultaneous Determination of Gastrodine and Guanosine in Banxia Baizhu Tianmatang[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 44-47.
DOI：

WANG Xun, LU Jia-feng, LUO Shan-shan, et al. Simultaneous Determination of Gastrodine and Guanosine in Banxia Baizhu Tianmatang[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 44-47. DOI：

摘要

目的: 建立同时测定半夏白术天麻汤中天麻素和鸟苷含量测定的方法。方法: 使用Platisil ODS 色谱柱(4.6 mm×250 mm

5 μ m)柱;流动相A(水)-B(乙腈);梯度洗脱0~30 min(2%~5%B);流速0.65 mL ·min-1;柱温25 ℃;检测波长260 nm。结果: 天麻素和鸟苷分别在0.007~0.35 g ·L-1(r=0.999 9)和0.000 32~0.016 g ·L-1(r=0.999 9)内线性关系良好

平均回收率分别为99.7%和100.6%

RSD分别为1.9%和2.0%(n=9)。结论: 所建立的方法准确快速

可用于半夏白术天麻汤复方的质量控制。

Abstract

ObJective: To establish an HPLC method for the content determination of gastrodine and guanosine in Banxia Baizhu Tianmatang. Method: The Platisil ODS-C18 column was used. The mobile phases consisted of water and acetonitrile with gradient elution. The flow rate was 0.65 mL ·min-1 and column temperature was at 25 ℃.The detection wavelength was at 260 nm. Result: Gastrodine and guanosine were linear in the range of 0.007-0.35 g ·L-1 (r=0.999 9) and 0.000 32-0.016 g ·L-1 (r=0.999 9) respectively. The average recoveries were 99.7% and 100.6% with the RSD 1.9% and 2.0% (n=9) respectively. Conclusion: The method was accurate and quick

and can be used for quality control of Compound Pinelliae and Atractylodis Macrocephalae and Grastrodiae Decoction.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Determination of Three Glycosides and Gastrodine Contents in Gastrodiae Rhizoma by RP-HPLC

Establishment of Quality Control Method in Production of Jinshuibao Capsules

Quantitative and Fingerprint of Ilex pubescens Slices

Content Comparison of Mannose, Glucose and Narinhenin in Dendrobium officinale and Dendrobium huoshanense

Experimental Studies on Quality Analysis of Jingyin Granule

Related Author

LIU Hong

CHEN Yan-qin

LUO Shu-chang

XIA Zhi

CAI Li

Xue-ting ZHANG

Cheng-zan ZHONG

Ming YANG

Related Institution

Key Laboratory of Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Jiangxi Guoyao Co. Ltd.

Jilin Agricultural University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Chengde Medical University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰