Purification of Total Flavones from  by Macroporous Resins

XIAO Ting; YAN Zhi-yong; ZUO Chang-ying; PAN Ling-zhen; ZHANG Zhan-ping

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Purification of Total Flavones from by Macroporous Resins

更新时间：2024-01-04

- Purification of Total Flavones from by Macroporous Resins
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 17, Pages: 36-39(2010)
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- Published：2010
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XIAO Ting, YAN Zhi-yong, ZUO Chang-ying, et al. Purification of Total Flavones from by Macroporous Resins[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 36-39.
DOI：

XIAO Ting, YAN Zhi-yong, ZUO Chang-ying, et al. Purification of Total Flavones from by Macroporous Resins[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 36-39. DOI：

摘要

目的: 研究蜘蛛香总黄酮的大孔树脂纯化工艺条件。方法: 采用静态吸附-解吸方法

以吸附量和解吸率为指标

筛选最佳树脂;以总黄酮收率为指标

考察最佳树脂纯化蜘蛛香总黄酮的工艺参数。结果: 5种树脂中

HPD600大孔树脂对蜘蛛香总黄酮纯化效果较好

其总黄酮的静态饱和吸附容量为131.11 mg ·g-1干树脂

解吸率为91.69%;最佳动态吸附、洗脱条件为蜘蛛香总黄酮提取液质量浓度3.42 g ·L-1

吸附流速2.4 BV ·h-1

上样量25.64 mg ·g-1干树脂;吸附后的树脂柱先以3 BV水洗脱

再用4 BV 80%乙醇以2.4 BV ·h-1流速洗脱

总黄酮质量分数为41.20%

收率为80.36%。结论: HPD600大孔树脂在所确定的工艺条件下

可较好的分离纯化蜘蛛香总黄酮。

Abstract

ObJective: To study the optimal technologies for the purification of total flavones from Valeriana Jatamansi with macroporous resin. Method: Taking the static elution ratio and absorption ratio as indexes

the static absorption-desorption was used and compared in order to find the optimum macroporous resin.The technical process for purification of total flavones with the optimum macroporous resin was screened by yield of total flavones as index. Result: The HPD600 macroporous resin among five types of resins was found with the best separating efficiency

by which static absorption capacity and desorption ratio could reach 131.11 mg ·g-1 and 91.69%

respectively. The best result of dynamic absorption was based on the followings: sample concentration 3.42 g ·L-1

feed rate 2.4 BV ·h-1

sample capacity 25.64 mg ·g-1

elution flow rate 2.4 BV ·h-1.After eluted with 3BV of distilled water and 4 BV of 80% ethanol

the yield of total flavones was 80.36% and the purity was 41.20%. Conclusion: With definite technical process

the HPD600 macroporous resin could be used to purify the total flavonoids of V. Jatamansi.

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Related Institution

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