HPLC-ELSD Determination of Astragaloside Ⅳ in Fufangshiwei Tablets
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HPLC-ELSD Determination of Astragaloside Ⅳ in Fufangshiwei Tablets
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 10, Pages: 93-95(2010)
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Published:2010
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LI Dai-xiao, LV Ying-chao, LI Yun-xia. HPLC-ELSD Determination of Astragaloside Ⅳ in Fufangshiwei Tablets[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(10): 93-95.
DOI:
LI Dai-xiao, LV Ying-chao, LI Yun-xia. HPLC-ELSD Determination of Astragaloside Ⅳ in Fufangshiwei Tablets[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(10): 93-95.DOI:
HPLC-ELSD Determination of Astragaloside Ⅳ in Fufangshiwei Tablets
Objective: To establish an HPLC-ELSD method for the determination of astragaloside Ⅳ in Fufang Shiwei tablets. Method: Agilent C18 ( 4.6 mm ×150 mm
5 μm) column was used.The mobile phase consisted of acetonitrile - water (35∶65) and the flow rate was 1.0 mL·min-1; ELSD was used as the detector. Results:Astragaloside Ⅳ was linear in the range of 1.12-6.72 μg (r = 0.9999); The average recovery (n=9) was 98.94%. Conclusion: The method can be used for the quality control of Fufang Shiwei tablets.
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Related Institution
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