Content Determination of Cholic Acid in Yansukang Aerosol by HPLC-ELSD

JI Yan-yan; ZHOU Lin; GHANG Xin-quan; CHEN Bo

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Content Determination of Cholic Acid in Yansukang Aerosol by HPLC-ELSD

更新时间：2024-01-04

- Content Determination of Cholic Acid in Yansukang Aerosol by HPLC-ELSD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 10, Pages: 91-93(2010)
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- Published：2010
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JI Yan-yan, ZHOU Lin, GHANG Xin-quan, et al. Content Determination of Cholic Acid in Yansukang Aerosol by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(10): 91-93.
DOI：

JI Yan-yan, ZHOU Lin, GHANG Xin-quan, et al. Content Determination of Cholic Acid in Yansukang Aerosol by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(10): 91-93. DOI：

摘要

目的 :建立咽速康气雾剂中胆酸的含量测定方法。方法 :利用HPLC-ELSD 法

采用Cromasil C18色谱柱(4.6 mm×250 mm

5 μm);流动相甲醇-0.1%冰醋酸水溶液梯度洗脱;漂移管温度 75 ℃;氮气流速33 psi。结果 :胆酸对照品在0.6542~16.355 μg内

进样量与峰面积间的对数值呈良好的线性关系

r = 0.9998

平均回收率100.27%

RSD为2.13%。结论 :本方法结果准确

重复性好

可作为该制剂的含量测定方法。

Abstract

Objective: To establish the content determination method for cholic acid in Yansukang by HPLC-ELSD. Method: An HPLC method was applied with Kromasil-C18 column (4.6 mm×250mm

5 μm) by a gradient elution using A (methanol)-B (0.1% glacial acetic acid solution) as the mobile phase.The drift tube temperature was set at 75 ℃

and N2 pressure was 33 psi. Result: The linear range of cholic acid was 0.6542-16.355 μg;The correlation coefficient (r) was 0.999 8

and the average recovery was 100.27%

with RSD 2.13% ( n =6). Conclusion: The method is accurate and reproducible

and can be used to determine the content of cholic acid in Yansukang.

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