Objective: To determinate the contents of total flavonoids in the prepared medical herbs of Cyperi Rhizoma from different areas. Method: The contents of total flavonoids were detected by UV while rutin was used as controller and measurement wavelength was 507 nm. Result: The results showed that rutin had a good Linear relationship in the concentrations between 3.0 mg·L-1 to 50.0 mg·L-1 (r=0.999 8). The contents of total flavonoids in most areas were 0.81％-1.75％ except from Shanghai which was 0.42%. Conclusion: The contents of total flavonoids of the prepared medical herbs of Cyperi Rhizoma were higher and it may be used as one of quality index.