Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 14, Pages: 80-81(2010)
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Published:2010
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NING Kang-jian, FENG Hui-ping, HE Sheng-li. Determination of Caffeic Acid in PYJP by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 80-81.
DOI:
NING Kang-jian, FENG Hui-ping, HE Sheng-li. Determination of Caffeic Acid in PYJP by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 80-81.DOI:
Objective: To develop an HPLC method for determination of the content of caffeic acid in PYJP. Method: An C18 column was used as the chromatographic column.The mobile phase consisted of methanol-phosphate buffer (23 ∶77)
the flow rate was 1.0 mL·min-1.The UV detection wavelength was at 323 nm. Result: There was a good linear relationship between the concentration of caffeic acid and peak area value when the concentrations of caffeic acid was within the range of 0.072 96- 0.437 76 μg(r=0.999 9).The average recovery was 99.10%
RSD 1.22%(n=5). Conclusion: The method was accurate
sensitive
reproducible and may be used in the determination of caffeic acid in PYJP