Study the Bioassay Method of Informational Active Components Alignment of Scorpion
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Study the Bioassay Method of Informational Active Components Alignment of Scorpion
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 8, Pages: 94-95(2010)
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Published:2010
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WANG Jing-juan, ZHANG Gui-jun, LI Qi-yu. Study the Bioassay Method of Informational Active Components Alignment of Scorpion[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(8): 94-95.
DOI:
WANG Jing-juan, ZHANG Gui-jun, LI Qi-yu. Study the Bioassay Method of Informational Active Components Alignment of Scorpion[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(8): 94-95.DOI:
Study the Bioassay Method of Informational Active Components Alignment of Scorpion
SDS-PAGE法和Alpha Ease FC 4.0.0软件测定全蝎蛋白药效组分的蛋白质相对分子质量。 结果 :全蝎蛋白药效组分的蛋白质质量分数为50.37%;得到较好的全蝎蛋白药效组分的10条电泳谱带
相对分子质量分别为94.162 0
66.400 0
40.357 0
27.541 0
22.518 0
19.520 0
12.766 0
11.190 0
9.665 3
8.534 2 KDa。 结论 :这10条谱带是全蝎蛋白药效组分的特征谱带
可用于全蝎蛋白药效组分的鉴别。
Abstract
Objective: We studied the extracted method of informational active components alignment of scorpion
groped the best electrophoresis conditions
determined the molecular mass of informational active components alignment
assured the expressing characteristic of the electrophoretic bands
and set up the bioassay method of informational active components alignment of Scorpion. Method: We extracted scorpion by water and salt fractionation to obtain informational active components alignment
Coomassie brilliant blue method determined its protein content
and SDS-PAGE electrophoretic technique and Alpha Ease FC 4.0.0 software determined its protein molecular weight. Result: The protein content of informational active components alignment was 50.37%. There were ten bands in the informational active components alignment. Ten bands in the SDS-PAGE were 94.162 0
66.400 0
40.357 0
27.541 0
22.518 0
19.520 0
12.766 0
11.190 0
9.665 3
8.534 2 KD. Conclusion: These ten bands were the characteristic bands of informational active components alignment
and might be used to identify the informational active components alignment.