Effects and mechanisms of Weikangning on gastric cancer

ZHONG Wa; KAN Fang-ju; YU Zhong; MIN Cun-yun; LI Qing-ming

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Effects and mechanisms of Weikangning on gastric cancer

更新时间：2024-09-23

- Effects and mechanisms of Weikangning on gastric cancer
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 4, Pages: 109-113(2010)
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- Published：2010
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ZHONG Wa, KAN Fang-ju, YU Zhong, et al. Effects and mechanisms of Weikangning on gastric cancer[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 109-113.
DOI：

ZHONG Wa, KAN Fang-ju, YU Zhong, et al. Effects and mechanisms of Weikangning on gastric cancer[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 109-113. DOI：

摘要

目的 :观察健脾解毒散结中药胃康宁含药血清对胃癌细胞周期调控因子CyclinD1

CyclinD2

Cdk4(Cyclin dependant kinase4

CDK4)

Cdk6和p16INK4amRNA及其蛋白表达的作用。方法: 分别将高、中、低不同剂量的中药胃康宁制剂ig SD大鼠

制备含药血清

然后分别用不同剂量含药血清培养胃癌细胞;采用免疫组织化学SABC法及RT-PCR法检测各组胃癌细胞中CyclinD1

CyclinD2

Cdk4

Cdk6和p16INK4amRNA和蛋白的表达。结果: 免疫组化结果显示

与空白对照组比较

胃康宁高、中、低剂量组CyclinD1灰度值变化

无统计学意义(P>0.05);CyclinD2

Cdk4

Cdk6灰度值均有明显升高

而p16INK4a灰度值则显著降低(P<0.05

P<0.01);RT-PCR结果显示

胃康宁高、中、低剂量组CyclinD1 OPTDI值变化

无统计学意义(P>0.05);但能明显降低CyclinD2

Cdk4和Cdk6 OPTDI值

同时升高p16INK4a OPTDI值(P<0.05

P<0.01)。结论 :中药胃康宁可抑制胃癌细胞促细胞周期相关因子CyclinD2

Cdk4和Cdk6的表达

同时增强胃癌细胞周期抑制因子p16INK4a的表达

这可能是健脾解毒散结中药胃康宁抑制胃癌细胞生长的作用机制。

Abstract

Objective: To study the effects and mechanisms of Chinese herbal medicine—Weikangning on gastric cancer. Method: Total of 120 SD rats were divided into control group

high dose group

medium dose group and low dose group

and fed with natural saline

10 and 5 g/kg of Weikangning decoction respectively. The experimental animals were finally killed for the preparation of drug-containing serum. The gastric cancer cell MGC-803 was cultured with the drug-containing serum drawn from the rats in different groups. The expressions of CyclinD1

CyclinD2

Cdk4

Cdk6 and p16INK4a were detected with immunohistochemistry method-SABC. The expression of mRNA of CyclinD1

CyclinD2

Cdk4

Cdk6 and p16INK4a were detected with RT-PCR. Result: In high

medium and low dose group

the gray scales and the OPDTI value of CyclinD1 had no remarkable change;the gray scales of CyclinD2

Cdk4

Cdk6 and the OPDTI values of p16INK4a increased significantly

but the gray scales of p16INK4a and the OPDTI values of CyclinD2

Cdk4

Cdk6 decreased remarkably

compared with those in control groups. Conclusion: The Chinese herbal medicine Weikangning decoction can decrease the expressions of CyclinD2

Cdk4

Cdk6

increase the expression of p16INK4a. This effect may be involved in mechanism of gastric cancer cell growth inhibition gain by the Weikangning decoction.

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Related Author

ZHONG Wa

KAN Fang-ju

YU Zhong

MIN Cun-yun

LI Qing-ming

Zheng WEI

Ai-hua ZHANG

Ya-feng LI

Related Institution

The Second Affiliated Hospital of Sun Yat-sen University

广州中山大学附属第二医院中医科

Henan Academy of Chinese Medicine

Henan Provincial People's Hospital

Graduate School， Hebei University of Chinese Medicine

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