Objective: To inquire the dose-effect relationship of Chinese medicine prepared aconite root (PAT) on effect of reviving the Yang for resuscitation

and to provide the experimental basis for security administration. Method: Build the isolated frog heart failure model with low calcium Ren liquid and measure the myocardial contractile force of isolated frog heart failure model; Build mouse deficiency of kidney-Yang model with irrigating the stomach giving Hydrocortisone and measure plasma cyclic adenosine monophosphate (c-AMP) content

cyclic guanosine monophosphate (c-GMP) content

and the ratio of c-AMP and c-GMP

endothelinimmune(ET)

organs thymus、spleen coefficient of mouse. Result: There was direct correlation between dose of PAT and effect of changing growth rate of the myocardial contractile force within 2.5-20 g \5L-1; There was direct correlation between dose of PAT and effect of changing c-GMP

c-AMP/ c-GMP

organs thymus and spleen coefficient within 0.72-5.78 g \5kg-1 dose range;There was direct correlation between dose of PAT and effect of changing c-AMP within 0.72-7.22 g \5kg-1 dose range; There was direct correlation between dose of PAT and effect of changing c-AMP within 0.72-4.33 g \5kg-1 dose range. Conclusion: The effect of reviving the Yang for resuscitation was notable and showed dosage-effect relationship. The conclusion from these experiments could hint the range of 5.56-44.44 g \5d-1 as clinic dose range

or toxicity become apparent.