Effects of Aβ25-35 on the Expression of Toll-like Receptor 3 and Toll-like Receptor 4 in NG108-15 Cells

ZHAO Bao-sheng; MA Qun; AI Lu; WANG Xiu-li; LIU Yong-gang; LIU Yong

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Effects of Aβ25-35 on the Expression of Toll-like Receptor 3 and Toll-like Receptor 4 in NG108-15 Cells

更新时间：2024-09-23

- Effects of Aβ25-35 on the Expression of Toll-like Receptor 3 and Toll-like Receptor 4 in NG108-15 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 5, Pages: 178-180(2010)
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- Published：2010
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ZHAO Bao-sheng, MA Qun, AI Lu, et al. Effects of Aβ25-35 on the Expression of Toll-like Receptor 3 and Toll-like Receptor 4 in NG108-15 Cells[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 178-180.
DOI：

ZHAO Bao-sheng, MA Qun, AI Lu, et al. Effects of Aβ25-35 on the Expression of Toll-like Receptor 3 and Toll-like Receptor 4 in NG108-15 Cells[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 178-180. DOI：

摘要

目的: 研究Aβ刺激对小鼠神经细胞株NG108-15 Toll样受体(TLR)3、TLR4 mRNA表达的影响。方法: Aβ刺激NG108-15细胞

24 h后

取细胞培养上清液

ELISA法测定炎症因子TNF-α、IFN-β含量

荧光定量PCR法测定TLR3、TLR4 mRNA表达量的变化。结果: Aβ诱导NG108-15细胞分泌TNF-α、IFN-β的量较正常对照组明显增多(均P<0.01);Aβ可诱导NG108-15细胞TLR3、TLR4 mRNA表达增强

与空白对照组相比

均P<0.01。结论: Aβ刺激可体外诱导NG108-15细胞TNF-α、IFN-β分泌增多

诱导TLR3、TLR4 mRNA表达增强

提示Aβ致阿尔茨海默病(AD)作用可能与免疫性炎症及TLRs信号转导通路有关。

Abstract

Objective: To investigate the influences of Aβ25-35 on the TLR3 and TLR4 mRNA. Method: NG108-15 cell line was stimulated with Aβ25-35

the supernatant was collected 24 hours later and the inflammatory factors TNF-α and IFN-β were quantitatively measured

the changes in the mRNA expression of TLR3 and TLR4 were detected by real-time PCR. Result: Aβ induced the over-secreting of TNF-α、IFN-β and over-expression of TLR3 and TLR4 mRNA (P<0.01). Conclusions: Aβ induced the over-secreting of inflammatory factors and over-expression of TLR3 and TLR4

indicating the action of Aβ in causing AD disease might be affiliated with the immune inflammation and the TLRs signaling pathway.

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Related Author

ZHAO Bao-sheng

MA Qun

AI Lu

WANG Xiu-li

LIU Yong-gang

LIU Yong

WAN Qin

ZHANG Yong-xiang

Related Institution

School of Chinese Pharmacy,Beijing University of Chinese Medicine

Henan University of Traditional Chinses Medicine

Basic Medical Sciences College,Shandong University of Traditional Chinese Medicine

Pharmacy College,Shandong University of Traditional Chinese Medicine

Shandong Provincical Hospital of Traditional Chinese Medicine

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