Protective Effect of Cerebrospinal Fluid Containing Zhenganxifeng Decoction on PC12 cell Injury Induced by MPP

LI Cheng-chong; LI Jia-ming; WANG Gang; ZOU De-jia; GENG Yu-tao; LI Qiang; NIU Ying-cai

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Protective Effect of Cerebrospinal Fluid Containing Zhenganxifeng Decoction on PC12 cell Injury Induced by MPP

更新时间：2024-01-04

- Protective Effect of Cerebrospinal Fluid Containing Zhenganxifeng Decoction on PC12 cell Injury Induced by MPP
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 9, Pages: 134-137(2010)
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- Published：2010
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LI Cheng-chong, LI Jia-ming, WANG Gang, et al. Protective Effect of Cerebrospinal Fluid Containing Zhenganxifeng Decoction on PC12 cell Injury Induced by MPP[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(9): 134-137.
DOI：

LI Cheng-chong, LI Jia-ming, WANG Gang, et al. Protective Effect of Cerebrospinal Fluid Containing Zhenganxifeng Decoction on PC12 cell Injury Induced by MPP[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(9): 134-137. DOI：

摘要

目的 :探讨镇肝熄风汤载药脑脊液对甲基-苯基-吡啶离子(1-methyl-4- phenylpyridinium

MPP+)诱导的PC12细胞损伤的保护作用。方法 :不同浓度的镇肝熄风汤载药脑脊液预处理体外培养的PC12细胞后

加入MPP+诱导PC12细胞损伤模型。用MTT法检测PC12细胞活力

实时定量PCR检测Bax和Bcl-2 mRNA的表达

Western blot检测Bax和Bcl-2蛋白表达。结果 :MPP+处理72~96 h

与空白对照组比较

模型组细胞活力明显降低

Bcl-2 mRNA和蛋白表达显著下调

Bax mRNA和蛋白表达显著上调(P<0.05)。与模型组比较

不同浓度的镇肝熄风汤载药脑脊液细胞活力明显升高

Bcl-2 mRNA和蛋白表达显著上调

Bax mRNA和蛋白表达显著下调

差异具有统计学意义(P<0.05)。结论 :镇肝熄风汤载药脑脊液对MPP+诱导的PC12细胞具有保护作用

可能是通过上调Bcl-2和下调Bax的 mRNA和蛋白表达而发挥抑制PC12细胞凋亡的作用。

Abstract

Objective: To investigate the protective effect of cerebrospinal fluid containing Zhenganxifeng decoction (CSF-ZGXF) on pc12 cell induced by 1-methyl-4-phenylpyridinium ion (MPP+). Method: Neurotoxicity was induced by addition of MPP+ into PC12 cell cultures. And different doses of CSF-ZGXF were administrated 24 h before addition of MPP+ into PC12 cell cutrure. Cell viability

mRNA and protein of Bax and Bcl-2 expression was assayed by MTT

real-time PCR and western blot. Result: Optical density for PC12 cells treated with MPP+ for 48\|96 h was lower than that of the control group. The mRNA and protein expression of Bcl-2 was down-regulated and those of Bax was up-regulated in MPP+ groups compared with the control group. The mRNA and protein expression of Bcl-2 were up-regulated and those of Bax were down-regulated in CSF-ZGXF group compared with the MPP+ group. Conclusion: The results suggest that protective effect of CSF-ZGXF on PC12 cells may correlate with down-regulation of mRNA and protein expression of Bax and up-regulation of those of Bcl-2.

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