Objective: To establish a simple

accurate method of HPLC to determine reduced and total vitamin C from Rosa roxburghii. Method: The method was developed with the condition as follows:DiamonsilTM C18 colum(4.6 mm × 250 mm

5 μm)

methanol-0.1% phosphoric acid(5 ∶95)as the mobile phase with the speed of 1.0 mL ·min-1 and the detection wavelength was 225 nm. Reduced and total vitamin C was detected before and after DTT reduction respedively. Result : The linear range of determination for reduced vitamin C was 0.074 1~0.666 9 μg (r=0.999 9) with the average recovery of 102.64%

RSD 1.13%

and the linear range of determination for total vitamin C was 0.050 25~0.804 μg(r=0.999 9)with the average recovery of 98.32%

RSD 1.12%. Conclusion : The method was simple

specific

and suitable for the determination of reduced and total vitamin C in R. roxburghii. By using HPLC method to detect the vitamin C contents of R. roxburghii with the similar wildlife habitats and altitudes in Guizhou province

it was found they that the content of vitamin C had no difference

and their average content was 1 142.11 g ·L-1.