Effects of Huoxue Xifeng Recipe on Neuronal Apoptosis and the Expression of Bcl-2 and Bax Protein after Focal Cerebral Infarction in Rats
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Effects of Huoxue Xifeng Recipe on Neuronal Apoptosis and the Expression of Bcl-2 and Bax Protein after Focal Cerebral Infarction in Rats
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 3, Pages: 74-76(2010)
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Published:2010
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GAO Hong-li, LIU Zhao-chun, QU Xiao-lan. Effects of Huoxue Xifeng Recipe on Neuronal Apoptosis and the Expression of Bcl-2 and Bax Protein after Focal Cerebral Infarction in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(3): 74-76.
DOI:
GAO Hong-li, LIU Zhao-chun, QU Xiao-lan. Effects of Huoxue Xifeng Recipe on Neuronal Apoptosis and the Expression of Bcl-2 and Bax Protein after Focal Cerebral Infarction in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(3): 74-76.DOI:
Effects of Huoxue Xifeng Recipe on Neuronal Apoptosis and the Expression of Bcl-2 and Bax Protein after Focal Cerebral Infarction in Rats
Objective: To observe the effects of Huoxue Xifeng recipe (HXR) on neuronal apoptosis and the expression of Bcl-2 and Bax protein in brain tissue after focal cerebral infarction(FCI) in rats. Methods: 40 Wistar male rats were randomly divided into five groups: sham group
model group
HXR group(20
10
5 g·kg-1 as high
moderate and low dose respectively). The model of FCI was established based on the principle of photo-chemical initiation of thrombosis. Intragastric administration of HXR continued 7 days before modeling and stoped the administration 24 h after the modeling. TUNEL technique was used to examine neuronal apoptosis
immunohistochemical and confocal laser scanning microscope methods to examine the expression level of Bcl-2 and Bax protein in brain tissue. Results: Compared with the model group
the apoptosis of neural cells and the expression of Bax protein significantly decreased in the HXR groups. However
the expression of Bcl-2 protein and Bcl-2/Bax ratio increased significantly ( P <0.01 or P <0.05). Conclusion: HXR has a action of resisting neuronal apoptosis for FCI in rats
the mechanism of neuroprotection may be related to increase in Bcl-2 expression
decrease in Bax expression and increase in Bcl-2/Bax expression ratio.
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