Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS
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Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 2, Pages: 32-33(2010)
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Published:2010
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Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 32-33.
DOI:
Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(2): 32-33.DOI:
Chemical Composition Analysis of Dangguibuxue Decoction by HPLC-DAD-MS
Objective: To study chemical components of Dangguibuxue decoction. Methods :The compounds were separated on a Hypersil-Hypurity C18(150 mm×2.1 mm
5 μm) analytical column. The column temperature was set at 40 ℃. Mobile phase consisted of methanol and water using a gradient elution. The flow rate was set at 0.2 mL·min-1. UV spectra were obtained by scanning from 200 nm to 370 nm. The APCI-MS spectra were obtained in the positive ion mode scanning from m/z 100 to 650. Results :The peaks were identified by comparing their UV spectra
their molecular weights and their retention behaviors with data reported in the literature. The ten components seem to be tentatively identified. Conclusions: Because of providing UV and molecular mass as well as retention time
LC-DAD-MS is a powerful tool for the chemical analysis of Traditional Chinese Medicines.