Objective: To investigate the influences of Tangbikang on the serum nerve growth factor(NGF) concentration and NGF mRNA expression in sciatic nerve in rats with diabetic penipheral neuropathy (DPN). Method: 70 SD rats were randomly divided into two groups: normal control 10 and diabetic penipheral neuropathy 60 by weight. Diabetes was induced by intraperitoneal injection of STZ

55 mg·kg-1. The diabetic models were affirmed upon blood glucose ≥16.7 mmol·L-1. And the diabetic rats were randomly divided into five groups: model group

methycobal treatment group

Tangbikang different dose treatment groups. After drug administration for 8 days

all the rats were sacrificed at week 8; The blood serum was obtained and NGF were detected; The sciatic nerve NGF gene was detected by the Real-time fluorescence quantitative polymerase chain reaction (PCR). All statistical calculations were made using the SPSS16.0 statistical package. Result: After the treatment by the middle and high dose of Tangbikang

the NGF level (P<0.01

P<0.01)in the blood serum increased greatly. The expression of NGF gene incresed (P<0.01). Conclusion: Tangbikang can increase the NGF protein and gene expression to play an important role of peripheral nerve protection.