Studies on the Anti-angiogenic Mechanism of the Formula of Chinese Medicine Active Ingredients Combined with Small Dose Cisplatin in Mice of Hepatocellular Carcinoma

CHEN Tao; FU Ya-ling; GONG Zi-peng; DENG Li-rong; HU Yue-qin

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Studies on the Anti-angiogenic Mechanism of the Formula of Chinese Medicine Active Ingredients Combined with Small Dose Cisplatin in Mice of Hepatocellular Carcinoma

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- Studies on the Anti-angiogenic Mechanism of the Formula of Chinese Medicine Active Ingredients Combined with Small Dose Cisplatin in Mice of Hepatocellular Carcinoma
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 5, Pages: 157-160(2010)
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- Published：2010
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CHEN Tao, FU Ya-ling, GONG Zi-peng, et al. Studies on the Anti-angiogenic Mechanism of the Formula of Chinese Medicine Active Ingredients Combined with Small Dose Cisplatin in Mice of Hepatocellular Carcinoma[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 157-160.
DOI：

CHEN Tao, FU Ya-ling, GONG Zi-peng, et al. Studies on the Anti-angiogenic Mechanism of the Formula of Chinese Medicine Active Ingredients Combined with Small Dose Cisplatin in Mice of Hepatocellular Carcinoma[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(5): 157-160. DOI：

摘要

目的: 探讨中药有效成分组方(QHF:其中Q、H、F依次为"清热解毒"、"活血化瘀"、"扶正固本")联合小剂量化疗药物顺铂(DDP)抗小鼠H22肝癌血管生成的作用机理。方法: 48只BaLb/c小鼠右腋sc小鼠H22肝癌细胞建立荷瘤模型

随机分设为QHF复方组、小剂量顺铂组(DDP)、联合用药组(QHF+DDP)及生理盐水组(NS)共4组。以抑瘤率为指标观察各药物对荷瘤小鼠肿瘤生长的抑制作用;免疫组化方法检测各药物对小鼠肝癌组织中的血管内皮生长因子(VEGF)、表皮生长因子受体(EGFR)及基质金属蛋白酶-2(MMP-2)表达的影响。结果: QHF组、DDP组和联合用药组的瘤重均较NS对照组明显降低(P<0.01);联合用药组较QHF组明显降低(P<0.01)

抑瘤率分别为47.45%

63.11%和72.95%。QHF组、DDP组和联合用药组小鼠移植瘤组织内VEGF

MMP-2的表达较NS对照组明显降低(P<0.05

P<0.01及P<0.01);小鼠移植瘤组织内EGFR的表达较NS对照组明显降低(P<0.01

P<0.05及P<0.01);联合用药组与各单药组之间的差异无统计学意义(P>0.05)。结论: QHF复方与小剂量DDP均具有抗肝癌及抗肝癌血管生成的作用;QHF复方与小剂量DDP单独或联合应用抗血管生成作用的机制可能与其下调肝癌组织中VEGF

EGFR及MMP-2 的表达有关。

Abstract

Objective: To investigate the anti-angiogenic mechanism of QHF (a formula of Chinese medicine ingredients

abbreviation Q for Qingrejiedu

H for Huoxuehuayu and F for Fuzhengguben) combined with small dose chemotherapy drug cisplatin(DDP)in H22 mice of hepatocellular carcinoma. Method: The forty eight Balb/c mice were inoculated with cell suspension of mouse hepatocellular carcinoma cell line H22 under the right axillary skin to establish the solid tumor model. Then they were randomly divided into four groups as follows: QHF group

DDP group

QHF+DDP group and NS (normal saline) group. The inhibition rates of tumor growth in tumor-bearing mice by drugs as an indicator were observed. The expression of VEGF

EGFR and MMP-2 in the tissue of mice tumor was detected with immunohistochemisty. Result: The weight of tumor was significantly lower in QHF

DDP and QHF+DDP group than that of in NS group(P<0.01)

while the weight of mice tumor was significantly lower in QHF+DDP group than that of in QHF group. Their inhibition rate was 47.45%

63.11%

72.95%

respectively. The expression of VEGF and MMP-2 in the tissue of mice tumor was significantly lower in QHF

DDP and QHF+DDP group than that of in NS group(P<0.05

P<0.01 and P<0.01). Moreover

The expression of EGFR in the tissue of mice tumor was significantly lower in QHF

DDP and QHF+DDP group than that of in NS group(P<0.01

P<0.05 and P<0.01). However

the difference between QHF+DDP group and other groups has no significance(P>0.05). Conclusion: Both of QHF and small dose DDP have the anti-tumor and anti-angiogenic effect in H22 mice of hepatocellular carcinoma. Moreover

one of anti-angiogenic mechanism may be concerned with the downregulation of the expression of VEGF

EGFR and MMP-2 in the tissue of mice tumor.

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Related Author

CHEN Tao

FU Ya-ling

GONG Zi-peng

DENG Li-rong

HU Yue-qin

Rui DING

Xiang WANG

Peng ZHOU

Related Institution

Medical College,Three Gorges University

Anhui University of Chinese Medicine

Dongzhimen Hospital， Beijing University of Chinese Medicine

The First School of Clinical Medicine， Shaanxi University of Chinese Medicine

Affiliated Hospital of Shaanxi University of Chinese Medicine

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