Determination of Tetrahydropalmatine in Yuanhuzhitong Oral Liquor by HPLC

LIU Rui-xin; WANG Qing-xiao

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Determination of Tetrahydropalmatine in Yuanhuzhitong Oral Liquor by HPLC

更新时间：2024-01-04

- Determination of Tetrahydropalmatine in Yuanhuzhitong Oral Liquor by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 8, Pages: 64-66(2010)
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- Published：2010
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LIU Rui-xin, WANG Qing-xiao. Determination of Tetrahydropalmatine in Yuanhuzhitong Oral Liquor by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(8): 64-66.
DOI：

LIU Rui-xin, WANG Qing-xiao. Determination of Tetrahydropalmatine in Yuanhuzhitong Oral Liquor by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(8): 64-66. DOI：

摘要

目的: 建立一种测定元胡止痛口服液中延胡索乙素含量的高效液相色谱方法。方法: 收集10批元胡止痛片

经过全面、规范的方法学验证

确定色谱条件为:C18柱(4.6 mm×250 mm

5 μm);流动相为乙腈-0.1％磷酸溶液(三乙胺调pH 6.0)(40 ∶60);检测波长280 nm。结果: 延胡索乙素在5.09~71.23 μg ·mL-1时

质量浓度与峰面积积分值呈良好的线性关系

r＝0.999 97

平均回收率101.28％

RSD2.05％;含量限度定为每1 mL含延胡索乙素不得少于80 μg。结论: 该方法简便、快速、准确

可用于元胡止痛口服液中延胡索乙素的含量控制。

Abstract

Objective: To establish a HPLC method for determination of tetrahydropalmatine in Yuanhuzhitong Oral Liquor. Method: 10 batches of Oral Liquor were collected. The HPLC determination method was established after comprehensive and standardized methodology validation: C18 column(4.6 mm×250 mm

5 μm) was used. The mobile phase was acetonitrile-0.1% phosphoric acid solution(adjusted to pH6.0 with triethylamine) (40 ∶60). The detecting wavelength was 280 nm. Result: The linear detection ranges of tetrahydropalmatine were 5.09~71.23 μg ·mL-1 (r＝0.999 97)

and the average recovery was 101.28％(RSD 2.05％); The content of tetrahydropalmatine shall not be less than 80 μg per mL. Conclusion: This method is convenient

rapid and accurate

which could be used for quality control in Yuanhuzhitong Oral Liquor.

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Related Author

DENG Yue-ting

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LU Ying

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Related Institution

National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine， Jiangxi University of Chinese Medicine

Jiangxi Bencao Tiangong Technology Co. Ltd.

Jiangxi Jiangzhong Traditional Chinese Medicine Decoction Pieces Co. Ltd.

Jiangxi Jinfurong Pharmaceutical Co. Ltd.， CSPC Pharmaceutical Group Co. Ltd.

School of Pharmacy, Anhui University of Chinese Medicine

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