The Cytotoxicity of the Effective Components of Maimendong Recipe and Qianjinweijing Recipe to Human Non-Small Cell Lung Carcinoma Cell Line H460

ZHENG Lu-yu; XIONG Fei; ZHAN Zhen; TANG Yu-ping; DUAN Jin-ao; ZHANG Xu

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The Cytotoxicity of the Effective Components of Maimendong Recipe and Qianjinweijing Recipe to Human Non-Small Cell Lung Carcinoma Cell Line H460

更新时间：2024-01-04

- The Cytotoxicity of the Effective Components of Maimendong Recipe and Qianjinweijing Recipe to Human Non-Small Cell Lung Carcinoma Cell Line H460
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 3, Pages: 60-63(2010)
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- Published：2010
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ZHENG Lu-yu, XIONG Fei, ZHAN Zhen, et al. The Cytotoxicity of the Effective Components of Maimendong Recipe and Qianjinweijing Recipe to Human Non-Small Cell Lung Carcinoma Cell Line H460[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(3): 60-63.
DOI：

ZHENG Lu-yu, XIONG Fei, ZHAN Zhen, et al. The Cytotoxicity of the Effective Components of Maimendong Recipe and Qianjinweijing Recipe to Human Non-Small Cell Lung Carcinoma Cell Line H460[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(3): 60-63. DOI：

摘要

目的: 通过药效评价实验筛选出麦门冬汤合千金苇茎汤(简称金方)的有效部位。方法: MTT法观察不同浓度的金方提取出的8个部位对肺癌NCI-H460细胞和正常人胚肺成纤维细胞HFL-I生长增殖的影响

初步筛选有效部位;平板细胞克隆形成试验

检测有效部位对肿瘤细胞克隆形成能力的影响;同时用流式细胞仪进行细胞周期检测分析

评价有效部位对细胞周期的影响;Hoechst33258染色后在荧光显微镜下观察NCI-H460细胞凋亡的形态变化。结果: 不同浓度提取各部位中

6部位对NCI-H460细胞有显著的生长抑制作用;作用72 h后

荧光显微镜下可见NCI-H460细胞核固缩

染色质凝聚等凋亡形态学特征;流式检测显示对NCI-H460细胞主要抑制在S期

并有凋亡细胞出现。结论: 麦门冬汤合千金苇茎汤提取各部位中6部位对NCI-H460细胞具有细胞毒作用

可有效抑制肺癌细胞生长

并对人体正常细胞(胚肺成纤维细胞)无显著影响

可以进一步分离筛选组分及单体化合物。

Abstract

Object: To screen the effective components of Maimendong Recipe and Qianjinweijing recipe with the efficacy evaluation. Methods: The effective parts which were extracted from these recipes by chemical methods

lung cancer H460 cell line and HFL-I cell line were cultured as model and assayed by MTT method respectively for the preliminary screen. Cell lines colony formation capacity was measured by monoclonality as well as cell cycle was analyzed by flow cytometry to estimate the screened components.Cell apoptosis was shown by Hoechst 33258 staining in cell morphology. Results: Extraction 6 can inhibit the proliferation of lung cancer cells significantly without influence on fibroblast cells’ activity(HFL-I). Conclusions: Extraction 6 of Maimendong recipe and Qianjinweijing recipe are the effective components against lung cancer cells

can be isolated and screened with further studies.

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