Effects of different proportion of donor splenocytes on chimerism and GVHD after haploidentical MHC matched stem cells transplant with nonmyeloablative conditioning in murine model

PENG En-lan; ZUO Hong-li; YU Chang-lin

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Effects of different proportion of donor splenocytes on chimerism and GVHD after haploidentical MHC matched stem cells transplant with nonmyeloablative conditioning in murine model

更新时间：2024-09-23

- Effects of different proportion of donor splenocytes on chimerism and GVHD after haploidentical MHC matched stem cells transplant with nonmyeloablative conditioning in murine model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 4, Pages: 155-159(2010)
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- Published：2010
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PENG En-lan, ZUO Hong-li, YU Chang-lin. Effects of different proportion of donor splenocytes on chimerism and GVHD after haploidentical MHC matched stem cells transplant with nonmyeloablative conditioning in murine model[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 155-159.
DOI：

PENG En-lan, ZUO Hong-li, YU Chang-lin. Effects of different proportion of donor splenocytes on chimerism and GVHD after haploidentical MHC matched stem cells transplant with nonmyeloablative conditioning in murine model[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(4): 155-159. DOI：

摘要

目的: 采用小鼠非清髓单倍体造血干细胞移植移植物抗宿主病(GVHD)模型

将单倍体相合供鼠的骨髓细胞和脾细胞混合移植给受鼠

探讨脾细胞数量对嵌合体形成及GVHD的影响。方法: 30只受鼠CB6F1(H2Kd/b)分为A

C组

均接受60Co 4Gy全身照射

实验组4~6 h后混合输入供鼠C57BL/6(H2Kb)脾细胞(依组次分别输注1×107

3×107

6×107个)和骨髓细胞(1×107个)。测定受鼠嵌合体水平

观察各组GVHD临床表现

进行评分

并观察生存期。结果: A及B组移植后3周达完全供者植入

C组于移植后2周达完全供者植入。A组小鼠未观察到GVHD表现

B组小鼠全部出现GVHD

其中部分小鼠有严重GVHD

C组小鼠均出现严重GVHD。各组小鼠临床GVHD评分有显著差异(各组间 P <0.01)

且C组＞B组＞A组。移植后全部存活超过90 d。结论: 增加小鼠单倍体造血干细胞移植物中脾细胞数量

有利于促进尽早形成完全供者嵌合体

但同时增加GVHD发生的可能性及严重程度。

Abstract

Objective: To explore the effects of splenocytes on chimerism and GVHD after haploidentical MHC matched stem cells transplant with nonmyeloablative conditioning in murine model. Method: Thirty CB6F1 recipient mice were divided into group A

B and C. They were given 60Co of total body irradiation and infused with MHC haploidentical matched bone marrow plus different proportion of splenocytes. The level of the chimerism of the recipient mice was detected by flow cytometry. The manifestations and histopathological examination of GVHD were observed. The mortality of the recipient mice was observed. Result: Engraftments of group A and B were full donor chimerism after 21 days

engraftments of group C were full donor chimerism after 14 days. The clinical GVHD score in group C was obviously higher than that in group B(P<0.01)

and that in group B higher than in group A(P<0.01). All the mice survived over 90 days. Conclusion: The higher dose of MHC haploidentical matched splenocytes will help to be full donor chimerism earlier

but it may aggravate GVHD.

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Effects of different proportion of donor splenocytes on chimerism and GVHD after haploidentical MHC matched stem cells transplant with nonmyeloablative conditioning in murine model

Related Author

PENG En-lan

ZUO Hong-li

YU Chang-lin

Related Institution

Department of Hematology,Affiliated Hospital of Academy of Military Medical Sciences

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