Objective: To observe and compare anti-tumor affects of different reinforcing spleen and stomach recipes and discuss its function mechanism. Methods :Human esophagus cancer cell line Eca 109 and liver cancer cell line HepG2 were in vitro cultured.Peripheral blood mononuclear cells(PBMC) were isoluted and cultured. MTT and Alamar Blue method were used to detect cell growth. Inhibitory effect of Liujunzi decoction (S)

Sijunzi decoction (F)

Erchen decoction (T) on Eca109 and the HepG2 cells growth and activation effect on the PBMC were observed and compared. PBMC activation by S

F

T to Eca109 and the HepG2 cell survival influence were compared simultaneously. Results :The three recipes all can inhibit Eca109 and the HepG2 cells growth to various degree and in a dose-dependent manner. T function is strongest

S the next best The inhibitory action of three recipes on Eca109 cell stronger than to HepG2 cell

but there was no statistical difference. The three recipes all have activation function to PBMC in various degree

F is the strongest

S the next best. PBMC activated has the obvious inhibitory action to Eca109 and the HepG2 growth.Conclusion:The reinforcing spleen and stomach recipes including Liujunzi decoction

Sijunzi decoction

Erchen decoction may play its anti-Eca109 and the HepG2 cell effect through two ways

one is to suppress the tumour cell growth directly

the other is to adjust the immunity . T suppresses the tumour cell growth strongly

F activates the PBMC function significantly.