Determination of Shanzhiside Methylester in Preparations of  by HPLC

HE Xi-rui; LI Mao-xing; FAN Peng-cheng; WEI Li-li; ZHANG Ru-xue; JIA Zheng-ping

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Determination of Shanzhiside Methylester in Preparations of by HPLC

更新时间：2024-09-23

- Determination of Shanzhiside Methylester in Preparations of by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 6, Pages: 71-73(2010)
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- Published：2010
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HE Xi-rui, LI Mao-xing, FAN Peng-cheng, et al. Determination of Shanzhiside Methylester in Preparations of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 71-73.
DOI：

HE Xi-rui, LI Mao-xing, FAN Peng-cheng, et al. Determination of Shanzhiside Methylester in Preparations of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(6): 71-73. DOI：

摘要

目的 :建立独一味制剂中山栀苷甲酯的HPLC测定方法

为进一步控制独一味制剂的质量提供良好的参考。方法 :采用HPLC反向色谱柱Symmetry C18 (4.6 mm×150 mm

5 μm)

梯度洗脱

0~9 min乙腈-水(9 ∶91)

9~20 min乙腈-水(15 ∶85)

流速1.0 mL ·min-1

柱温20 ℃

检测波长235 nm。结果 :山栀苷甲酯浓度在9.6~115.2 μg ·mL -1范围内与峰面积积分值线性关系良好;加样回收率为97.69%。从所有的制剂中均检测出山栀苷甲酯

但含量差别较大。结论 :该方法准确、简便、专属性强、重现性好

适用于独一味制剂中山栀苷甲酯的含量测定。

Abstract

Objective: To establish a HPLC method for the determination of shanzhiside methylester in the preparations of Lamiophlomis rotata . Method: HPLC analysis was performed on Symmetry C18 (5 μm) column and gradient elution ∶91)

9-20 min

acetonitrile-water (15 ∶85)] was used as the mobile phase at a flow rate of 1.0 ml ·min-1. The column temperature was 20 ℃ and the detection wavelength was at 235 nm. Result: The linear range of shanzhiside methylester was 9.6~115.2 μg ·mL-1 (r=0.999 8) and the average recovery was 97.69%. Shanzhiside methylester could be detected from all preparations and the content variedgreatly. Conclusion: The method can be applied to determine the content of shanzhiside methylester in the preparations of Lamiophlomis rotata .

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Related Institution

College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine

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Hubei Institute for Food and Drug Control

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