Objective :To establish a HPLC method for simultaneous determination of chlorogenic acid and hesperidin in Zhike Ningsou Capsule . Method :The sample was extracted with methanol under ultrasonication. The HPLC separation was performed on a Zorbax SB C18 (3.0 mm ×250 mm

5 μm) column

using water containing 1% acetic acid(A) –acetonitrile(B) as the mobile phase

in stepwise elution:0 min(82 ∶18)~5 min(82 ∶18)~5.1 min(75 ∶25)~20 min(75 ∶25)

at a flow rate of 0.35 mL ·min-1. Result :The linear range was 0.4~10.0 μg ·mL-1 and 6.2~155.0 μg ·mL-1 for chlorogenic acid and hesperidin; the average recovery was 99.2% and 99.6%; RSD 1.0% and 0.9%

respectively. Conclusion :This method can be applied for the quality control of Zhike Ningsou Capsule.