RP-HPLC Determination of Quercetin and Kaempferol in the Leaves of Phyllanthus emblica L

LIANG Chen-yan; ZHEN Han-shen; MA Wen-Fang; LIU Yuan-yuan; CHEN Mei-an

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RP-HPLC Determination of Quercetin and Kaempferol in the Leaves of Phyllanthus emblica L

更新时间：2024-01-04

- RP-HPLC Determination of Quercetin and Kaempferol in the Leaves of Phyllanthus emblica L
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 8, Pages: 41-43(2010)
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- Published：2010
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LIANG Chen-yan, ZHEN Han-shen, MA Wen-Fang, et al. RP-HPLC Determination of Quercetin and Kaempferol in the Leaves of Phyllanthus emblica L[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(8): 41-43.
DOI：

LIANG Chen-yan, ZHEN Han-shen, MA Wen-Fang, et al. RP-HPLC Determination of Quercetin and Kaempferol in the Leaves of Phyllanthus emblica L[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(8): 41-43. DOI：

摘要

目的 :建立广西余甘子叶中槲皮素及山奈酚的含量测定方法。方法 :采用HPLC法

色谱柱为Thermo Hypersil BDS C18 (4.6 mm×250 mm

5 μm);流动相为乙腈-0.1%磷酸(27 ∶73);流速为1.0 mL·min-1;柱温为30 ℃;检测波长为360 nm。结果 :槲皮素在0.06~1.2 μg之间;山奈酚在0.021~0.43 μg

峰面积与进样量呈良好的线性关系;槲皮素和山奈酚的加样回收率分别为98.83%

101.58%

RSD分别为2.73%

2.21%(n=6)。结论 :该方法简便可行

重复性好

结果准确

可作为广西余甘子叶中槲皮素和山奈酚的含量测定方法。

Abstract

Objective: To establish a quantification method for quercetin and kaempferol in the leaves of Phyllanthus emblica by HPLC. Method: HPLC is applied on the determination of quercetin and kaempferol in the leaves of P. emblica with C18 column(4.6 mm×250 mm

5 μm) and a mixture of acetonitrile-0.1% phosphoric acid (27:73) as a mobile phase with the flow rate at 1.0mL·min-1. Quercetin and kaempferol were detected at 360nm

column temperature was set at 30 ℃. Result: Quercetin and kaempferol were linear in the range of 0.06~1.2 μg and 0.021~0.43 μg. The average recovery of quercetin and kaempferol was 98.83% with RSD 2.73% and 101.58% with RSD 2.21% (n=6) respectively. Conclusion: The method can be applied to the quality control of the leaves of P. emblica L.

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